Frequency of Th17 and Treg in HBV-related acute-on-chronic liver failure and its clinical value

Abstract

Abstract: Objective To investigate the frequency of peripheral Th17, Treg and Th17/Treg in patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF), and its correlation with disease progression and prognosis. Methods Seventy-four cases were enrolled, including 33 HBV-ACLF cases (HBV-ACLF group), 30 chronic hepatitis B cases (CHB group) and 11 healthy cases (control group). The frequency of Th17 and Treg cells and surface antigen quantity in peripheral blood were detected in three groups with flow cytometry technique, respectively. Correlation analysis between changes of Th17 and Treg frequency in the HBV-related ACLF cases and total bilirubin (TBiL), alanine aminotransferase (ALT) and prothrombin time (PT) was performed. Results Frequency of Th17, Treg cells and proportion of Th17/Treg were higher in HBV-ACLF patients than those in CHB and control group (P<0.05), respectively. In HBV-ACLF group, frequency of Th17 in dead patients were higher than that in survivors; frequency of Th17 and Th17/Treg were positively correlated with PT, ALT and AST (P<0.05) with no significant correlation with TBiL. Additionally, Th17/Treg was also positively correlated with alpha fetoprotein (AFP) (P<0.05). Conclusion There were different degrees of immune dysfunction in HBV-ACLF patients. Frequency of Th17 and Th17/Treg were associated with disease progression. Higher frequency of Th17 predicted worse prognosis, which revealed it might be important indicators for those patients.

Key words: Th17, Th17/Treg, ACHBLF, Disease progression, Prognosis

KAN Yan-ting, GAN Jian-he, SUN Wei, FENG Ting-ting. Frequency of Th17 and Treg in HBV-related acute-on-chronic liver failure and its clinical value[J]. Chinese Hepatolgy, 2016, 21(2): 89-91.

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