Abstract: Objective To review the clinical data of AILD patients who were first diagnosed in our hospital and met the inclusion criteria, and to explore the influence of Hp infection on the occurrence and development of AILD.Methods From 2015.9 to 2022.2, 74 patients with AILD first diagnosed were reviewed, including 43 males and 31 females, aged (58.2±12.5) years. The diagnosis of AIH, PBC and PSC met the standard. 14C-UBT and gastric antrum rapid urokinase test were used to understand the Hp infection. The first diagnosed AILD cases in Liaocheng Traditional Chinese Medicine Hospital were divided into groups according to the status of Hp infection or not, and the liver function, autoantibodies, lymphocyte subsets and pathological changes of liver tissues in different groups were compared.Results Among 74 patients with AILD, AIH, PBC and PSC were 34 cases (45.9%), 33 cases (44.6%) and 7 cases (9.5%), respectively. According to the results of 14C-UBT, they were divided into Hp positive group (44 cases, 59.5%) and Hp negative group (30 cases, 40.5%) in this study. In Hp positive group, ANA, AMA, SMA, ANCA, AMA-M2 and LKM-1 were 20 cases (45.4%), 18 cases (40.9%), 17 cases (38.6%), 23 cases (52.2%), 25 cases (56.8%) and 21 cases (47.9%), which were significantly higher than those in Hp negative group [6 cases (20.0%), 4 cases (13.3%), 4 cases (13.3%), 5 cases (16.7%), 2 cases (6.7%) and 2 cases (6.7%), P<0.05]. The percentages of CD3⁺CD4⁺T cells, CD4⁺/CD8⁺ and CD3^-CD19⁺B cells in Hp positive group were (42.1±6.2)%, (1.9±0.8) and (15.2±3.5)%, which were significantly higher than those in Hp negative group [(37.7±5.5)%, (1.4±0.5) and (11.9±2.5)%, respectively, P<0.05]. In Hp positive group, CD3⁺CD8⁺T cells and CD3^-CD16⁺CD56⁺NK cells were (25.4±5.0)% and (11.7±2.8)% in this study, which were significantly lower than [(29.0±5.1)% and (19.7±3.7)% respectively, P<0.05]. The proportion of Hp positive group [30 cases (68.2%)] ≥F3 was significantly higher than that of Hp negative group [13 cases (43.3%), P<0.05], at the same time, the former [24 cases (54.6%)] ≥ G3 grade was higher than the latter [8 cases (26.7%), P<0.05].Conclusion More than half of AILD patients are infected with Hp, which is closely related to the increase of various autoantibodies and the disorder of lymphocyte subsets, which can lead to the increase of liver fibrosis stage and inflammatory activity grade.

Key words: Autoimmune liver disease, Helicobacter pylori, Autoantibody, Lymphocyte subsets, Hepatic fibrosis staging, Inflammation activity grading