Chinese Hepatolgy ›› 2023, Vol. 28 ›› Issue (10): 1186-1190.

• Liver Cancer • Previous Articles     Next Articles

Analysis of serum levels and correlation of miR-504, miR-200a and IL-24 in patients with HBV-related hepatocellular carcinoma

CHENG Xiao-mei1, TIAN Hong2, LI Xiang1, YANG Jie3   

  1. 1. Department of Gastroenterology,Air Force 986th Hospital,Xi′an 710054,China;
    2. Department of Nephrology,Air Force 986th Hospital,Xi′an 710054,China;
    3. Department of Obstetrics and Gynecology,Air Force 986th Hospital, Xi′an 710054,China
  • Received:2023-05-23 Online:2023-10-31 Published:2023-12-06
  • Contact: YANG Jie, Email:yangjie451@126.com

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Abstract: Objective To investigate the serum levels of micro-RNA (miR) 504, miR-200a and IL-24, and thier correlation in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma. Methods We selected patients with HBV-related hepatocellular carcinoma who were treated in our hospital from February 2020 to February 2023 and met the inclusion and exclusion criteria as the observation group (N=60). The control group (N=60) consisted of patients with chronic HBV infection in the same period. The general data of the two groups were compared. The levels of serum miR-504 and miR-200a were measured by real-time fluorescence quantitative PCR, and the level of serum IL-24 was measured by enzyme-linked immunosorbent assay (Elisa). The risk factors associated with hepatocellular carcinoma in patients with chronic HBV infection were screened by correlation analysis and binary Logistic regression analysis. The diagnostic value of these factors was evaluated by the receiver working characteristic curve (ROC) and the area under the curve. Results The average HBV DNA load in the observation group was significantly higher than that in the control group (21.87 ±4.64 vs 13.61 ±3.36, P<0.05), but there was no significant difference in other general data. The serum levels of miR-504 (0.75±0.16 vs 1.09±0.20), miR-200a (0.79±0.24 vs 1.01±0.28) and IL-24 (187.65±19.04 vs 213.25±20.27) in the observation group were significantly lower than those in the control group. Correlation analysis and Logistic regression analysis showed that the decrease of serum miR-504, miR-200a and IL-24 levels were independent risk factors for hepatocellular carcinoma in patients with chronic HBV infection(P<0.05). ROC curve analysis showed that serum miR-504 had the highest diagnostic value for hepatocellular carcinoma in patients with chronic HBV infection (AUC=0.898, P<0.05). Conclusion The levels of miR-504, miR-200a and IL-24 in the serum of patients with HBV-related hepatocellular carcinoma showed a significant decrease. Moreover, each of these indicators exhibited a negative correlation with the occurrence of hepatocellular carcinoma in patients with chronic HBV infection. These findings suggest that the measurement of these indicators had a certain diagnostic value for HBV-related hepatocellular carcinoma.

Key words: Hepatitis B virus, micro-RNA, Interleukin-24, Hepatocellular carcinoma