Abstract: Objective To detect the characteristics of intestinal microecological structure changes in patients with hepatocellular carcinoma.Methods 87 patients with hepatocellular carcinoma were enrolled in this study. Another 80 healthy persons were taken as control. The microbial genomic was prepared for high throughput sequencing by Illumina platform. Results There was no significant difference in α diversity between hepatocellular carcinoma group and control group (P>0.05). The percentage of bacteroidetes and proteobacteria in hepatocellular carcinoma group was 56.41±4.63% and 9.26±1.82%, which was significant higher than that of 53.32±4.22% and 7.42±1.16% in control group(P<0.05 and P<0.01). The percentage of firmicutes and actinobacteria in hepatocellular carcinoma group was 32.62±3.75% and 0.34±0.05%, which was significant lower than that of 37.25±4.13% and 0.62±0.11% in control group(P<0.05 and P<0.01). The percentage of bacteroides and H.hepaticus in hepatocellular carcinoma group was 50.83±4.15% and 11.35±1.87%, which was significant higher than that of 42.45±3.84% and 8.52±1.71% in control group(P<0.01). The percentage of bifidobacterium and clostridium in hepatocellular carcinoma group was 21.13±3.64% and 10.44±1.25%, which was significant lower than that of 28.54±4.13% and 14.28±1.52% in control group(P<0.01).Conclusion The proportion of bacteroidetes, proteobacteria, bacteroides, and H.hepaticus in patients with hepatocellular carcinoma was significantly higher than that of control group. The proportion of firmicutes, actinobacteria, bifidobacterium and clostridium in patients with hepatocellular carcinoma was significantly lower than that of control group.

Key words: hepatocellular carcinoma, intestinal microecosystem, Illumina genome analyzer