Abstract: Objective To establish a magnetic bead adsorption method to quantitatively detect hepatitis B virus (HBV) DNA, and to evaluate its efficiency. Methods Serum template of 10⁷ IU/ml HBV virus was diluted into different concentrations as serum standard panels (theoretical value), HBV DNA in which were extracted by our magnetic bead adsorption method (reagent 1), domestic automatic nucleic acids extraction with magnetic beads (reagent 2) and boiling method (reagent 3), respectively. Comparisons in extraction efficiency among those 3 methods were carried out by analyzing sensitivity, quantitative linear relationship and stability. Results Theoretical value of lower HBV DNA detection limit was 10¹ IU/mL, and the extraction results were 3.520×10¹ IU/mL by reagent 1, 9.123×10³ IU/mL by reagent 2 and 6.195×10¹ IU/mL by reagent 3. Correlation analysis between extraction results and theoretical values showed r values as 0.986 (reagent 1, P<0.001), 0.950 (reagent 2, P=0.001) and 0.979 (reagent 3, P<0.001), respectively, which revealed statistically significant differences. Average relative deviations of the 3 methods were 0.243±0.405 (reagent 1), 1.189±0.855 (reagent 2), -0.439±0.618 (reagent 3), respectively. In addition, the average relative deviation of reagent 1 for same sample at different times was 0.505±0.659. Conclusion Magnetic bead adsorption method for HBV DNA extraction has good sensitivity, quantitative linear relationship, stability and accuracy, which might be a reliable method for the quantitative detection of HBV DNA.

Key words: HBV DNA, Extraction, Efficiency