Expression of IL-22 in hepatic steatosis intervened by blueberry probiotic serum

Abstract

Abstract: Objective To investigate mechanism of blueberry probiotic in treating nonalcoholic fatty liver disease (NAFLD) via affecting expression of interlukin-22 (IL-22).Methods Serum from rats treated with saline, and low, medium and high dose of blueberry probiotic by gavage were collected, respectively. Cell steatosis model was constructed by stimulating normal liver cell line L-02 with free fatty acid (FFA). Serum from different dose blueberry probiotic treated rats were added into cell culture medium as 10% concentration for the L-02 steatosis model, respectively. Intracellular lipid deposition was observed by oil red O staining and level of triglyceride (TG) was quantitatively determined. Gene expression of IL-22 was detected by reverse transcription polymerase chain reaction (RT-PCR) and protein expression of IL-22 was detected by western blot and immunofluorescence.Results After 24-hour FFA stimulation in L-02 cell with serum, a pile of lipid deposition could be observed and TG level was significantly increased (P=0.000), while mRNA and protein levels of IL-22 were decreased (P=0.000). In blueberry probiotic serum groups, TG levels and lipid depositions were negatively correlated with the dose of blueberry probiotic. In high-dose blueberry probiotic serum group, expression of IL-22 was significantly enhanced comparing with the other groups (P=0.000). However, there was no significant difference in expression of IL-22 between the low- and medium-dose serum groups (P>0.05).Conclusion Blueberry probiotic might play a role in treatment of NAFLD by enhancing the expression of IL-22.

Key words: Blueberry probiotic serum, Hepatocyte, Steatosis, Interleukin-22

ZHU Juan-juan, CHENG Ming-liang. Expression of IL-22 in hepatic steatosis intervened by blueberry probiotic serum[J]. Chinese Hepatolgy, 2016, 21(8): 630-635.

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