Abstract: Objective To investigate the distribution and drug resistance of pathogen in patients with biliary tract infection, and to guild clinical application of antibiotics.Methods The cases of biliary tract infection with positive bile pathogen culture in our hospital from September 2014 to August 2016 were retrospectively analyzed, and the distribution and drug resistance of 143 strains of pathogen from those cases were statistically analyzed using WHONET 5.4.Results Among the 143 strains of pathogen, there were 77 (53.8%) gram-negative bacilli, 63 (44.1%) gram-positive coccus and 3 (2.1%) fungal strains. The most common pathogenic bacteria in biliary tract infection were Enterococcus faecalis (12.6%, 18/143), Enterococcus faecium (11.9%, 17/143), Klebsiella pneumoniae (11.2%, 16/143), Escherichia coli (9.1%, 13/143) and Pseudomonas aeruginosa (8.9%, 12/143). The susceptibility tests revealed that resistant rates of gram-negative bacilli against amikacin and doripenem were fairly low, and of gram-positive coccus against linezolid, daptomycin and vancomycin were also fairly low.Conclusion In biliary tract infection, the infectious rate of gram-positive coccus increased year by year, though the rate of gram-negative bacilli took the lead invariably. Clinical selection of antibiotics should be more reasonable and specific, as the pathogen developed resistance to the most common antibiotics obviously.

Key words: Bile, Pathogenic bacteria, Drug resistance