Abstract: Objective To investigate the efficiency of salting-out and frozen-thaw method for extracting cryoprecipitate from plasma in hepatitis patients for retransfusion therapy.Methods A total of 200 mL plasma from patients with severe hepatitis were diluted with equal volume of half-saturated sterile NaCl solution. Then the diluted plasma was frozen at -30 ℃ for at least 48 h or at -80 ℃ for 6 h. At -4 ℃, the frozen plasma was thawed and centrifugated at 500 g for 10 min. Removing the supernatant, the sediment was dissolved with acid citrate dextrose (ACD) solution to 20 mL cryoprecipitate (CP) solution. The values of Na⁺, Cl^－, fibrinogen (FIG), fibronectin (Fn), factor Ⅷ, prothrombin time (PT) and activated partial thromboplastin time (APTT) of the plasma and CP solution were measured, respectively. .Results Compared with plasma, CP solution showed 2~3 times higher levels of Na⁺ and Cl^－, 7 times higher levels of FIG and Fn, and nearly 9 times higher level of factor Ⅷ. However, CP solution showed lower levels of PT and ATPP than the plasma, with no significant difference (P>0.05 ).Conclusion The salting-out and frozen-thaw method could be used to extract CP from plasma, and the extracted CP should be diluted properly for further retransfusion.

Key words: Cryoprecipitate, Hepatitis, Salting-out, Back transfusion