Chinese Hepatolgy ›› 2017, Vol. 22 ›› Issue (4): 310-313.

• Original Articles • Previous Articles     Next Articles

Expression of miRNA-21/101/125a/195/200b in hepatic cirrhosis and carcinoma

LEI Yang1, TAO Yan-yan1, WANG Qing-lan2, LUO Yun-quan3, LIU Cheng-hai1   

  1. 1. Institute of Liver Diseases, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
    2. E-Institute of Traditional Chinese Medicine Internal Medicine, Shanghai Municipal Education Commission, Shanghai 201203, China;
    3. Department of Hepatobiliary Surgery, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
  • Online:2017-04-30 Published:2017-04-30
  • Contact: LIU Cheng-hai, Email: chenghailiu@hotmail.com

PDF (PC)

354

Abstract: Objective To investigate the differential expression of microRNA (miRNA, miR) 21/101/125a/195/200b in patients with hepatic cirrhosis and hepatocellular carcinoma (HCC). Methods Liver samples of hemangioma (6 cases), cirrhosis (10 cases) and HCC and adjacent tissues (14 pairs) were collected, in which hepatitis B surface antigen (HBsAg) was positive except samples from hemangioma. Real-time polymerase chain reaction (RT-PCR) was applied to detect the expression of miR-21/101/125a/195/200b, respectively. Pearson correlation analysis was carried out between expression of miR-101 and the related clinical indexes. Results In cirrhosis group, levels of prothrombin time (PT), international normalized ratio (INR), aspartate transaminase (AST) and serum total bilirubin (TBiL) were significantly higher than those in hemangioma group, while blood platelet (PLT) was significantly lower. Hematoxylin and eosin (HE) staining, Masson staining and reticular fiber staining were all consistent with the diagnosis of cirrhosis. In HCC group, white blood cell (WBC) and PLT were increased, while levels of PT, INR, AST and TBil were decreased compared with those in cirrhosis group. Using immunohistochemical staining, alpha fetal protein was significantly higher in HCC group than that in hemangioma or cirrhosis groups, which was confirmed the diagnosis of HCC in turn. Compared with hemangioma group, miR-101 and miR-195 were down-regulated in cirrhosis group. Compared with the para-carcinoma tissues, miR-21 was up-regulated in HCC tissue, while miR-101 and miR-195 were down-regulated. There were no statistical difference in the expression of miR-125a and miR-200b among the three groups. miR-101 was positively correlated with the expression of PLT with coefficient of 0.375. Conclusion miR-101 is remarkably decreased in both HBV-related cirrhosis and HCC, which is positively correlated with PLT. It suggests that miR-101 might be a negative regulator of chronic liver disease.

Key words: microRNA-101 (miRNA-101, miR-101), Hepatic cirrhosis, Hepatocellular carcinoma (HCC), Chronic hepatitis B (CHB), Liver fibrosis