Effect of nonalcoholic fatty liver disease on diversity of intestinal flora

Abstract

Abstract: Objective To investigate the effect of nonalcoholic fatty liver disease (NAFLD) on the diversity of intestinal flora.Methods The NAFLD rat model was established by feeding high fat diet for 18 weeks and validated by liver histological examination. Fecal microbial flora was detected by 16sRNA sequencing technique. Results Liver histology showed bullae and microvesicular fatty degeneration and ballooning, which suggested NAFLD rat model was successfully constructed. Shannon index of control group was significantly higher than that of NAFLD group (P<0.05), suggesting that the microbial diversity in control group was significantly higher than that in NAFLD group. Changes at phylum level of intestinal flora revealed that the relative abundance of Firmicutes and Proteobacteria in NAFLD group were significantly higher (P<0.05) than that in control group, while the relative abundance of Bacteroidetes is significantly lower (P<0.05). Changes at family level of intestinal flora showed that the dominant bacteria were Lachnospiraceae, Ruminococcaceae and Bacteroidales_S24-7_group in control group, while Lachnospiraceae and Ruminococcaceae in NAFLD group. Compared with those in control group, the relative abundance of Lachnospiraceae, Bacteroidaceae, Desulfovibrionaceae, Acidaminococcaceae and Christensenellaceae were significantly higher (P<0.05) in NAFLD group, while the relative abundance of Bacteroidales_S24-7_group, Prevotellaceae and Lactobacillaceae were significantly lower (P<0.05). Conclusion NAFLD can impair the diversity of intestinal flora and significantly change the composition and content of intestinal flora.

Key words: Nonalcoholic fatty liver disease, Intestinal flora, Diversity

ZHANG Jing-yi,TANG Ying-yue,LI Chun-min,MING Ya-nan,TANG Jie-ting,ZENG Min-de,MAO Yi-min. Effect of nonalcoholic fatty liver disease on diversity of intestinal flora[J]. Chinese Hepatolgy, 2017, 22(4): 323-326.

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