Three methods to identify hepatitis B virus strains with C/D recombinant

Abstract

Abstract: Objective To investigate accurate method for identifying hepatitis B virus strains with C/D recombinant.Methods A total of 25 patients with chronic hepatitis B from western China were enrolled in this study. Three methods, containing polymerase chain reaction (PCR) of fragment length analysis, phylogenetic analysis based on S and preC/C gene, and full genome precise analysis, were compared.Results On account of the 33bp deletion in preS1 of genotype D, a PCR fragment spanning the preS1 region could identify C/D recombinant from genotype D, which was easy to perform and suitable for large sample screening. Phylogenetic analysis based on S and preC/C gene could identify C/D recombinant with high accuracy. Full genome precise analysis could identify C/D recombinant precisely and accurately which was the most reliable method.Conclusion For various research purposes, the application of the 3 methods should be performed comprehensively to improve the efficiency and reliability in identification of C/D recombinant.

Key words: Hepatitis B virus, Genotype, C/D recombinant

LI Ji-jie, BAO Cheng-lan, GAMA Cai-pei, WANG Yun, LI Wei, ZHOU Bin, WANG Zhan-hui, LI Hua. Three methods to identify hepatitis B virus strains with C/D recombinant[J]. Chinese Hepatolgy, 2017, 22(8): 695-699.

[1]	WANG Juan-juan, SHI Hong-ying, ZHENG Shi-tan. The clinical characteristics of 11 cases of systemic lupus erythematosus with non primary biliary cirrhosis [J]. Chinese Hepatolgy, 2020, 25(5): 491-493.
[2]	HUANG Shan-shan, ZHAO Jing-ya, CHEN Jie, YANG Yang.. The study on mechanism of prostaglandin E2 promoting replication of hepatitis B virus [J]. Chinese Hepatolgy, 2020, 25(3): 277-281.
[3]	WANG Xian, LIN Tao-fa, ZHAN Zhi-yu, WANG Shao-yang. Differential expression of T lymphocyte and NK G2D in patients with chronic HBV infection in different immune states [J]. Chinese Hepatolgy, 2020, 25(2): 131-134.
[4]	HE Ya-jing, ZHANG Zhi-ming, HE Wei-meng, HOU Jin-lin. Efficacy of different anti-viral therapy regimens for HBV related hepatocellular carcinoma receiving radiotherapy [J]. Chinese Hepatolgy, 2019, 24(6): 622-627.
[5]	ZHAO Jing-jing, HAN Fang-zheng. Clinical Study of transient ALT elevation in patients with chronic hepatitis B in the early stage of entecavir treatment [J]. Chinese Hepatolgy, 2019, 24(6): 628-630.
[6]	SU Jin-liang, CHEN Hui, ZHU Li, LU Min-Jia, LI Ming. Expression of toll-like receptor 3 and 4 in peripheral blood mononuclear cells from patients with hepatitis B virus -related acute-on-chronic pre-liver failure [J]. Chinese Hepatolgy, 2019, 24(5): 499-502.
[7]	CAO Xiang-yun, Guan Yan-ping, LI Qian, ZHOU Xiao-min, LIU Gao-wei. Comparison of the effects of tenofovir and telbivudine combined with dual immunization regimen on blocking mother-to-child transmission of HBV during pregnancy [J]. Chinese Hepatolgy, 2019, 24(5): 503-506.
[8]	LI Rong, ZHANG Hang, HUANG Xi-yao, DU Dan. The analysis on association between IL28B single gene polymorphisms and the risk of intrauterine infection of HBV in HBsAg positive pregnant women [J]. Chinese Hepatolgy, 2019, 24(3): 234-238.
[9]	GU Jing, CHEN Zu-tao, SUN Wei, GAN Jian-he. Changes of serum alkaline phosphatase in patients with HBV-related acute-on-chronic liver failure and its significance [J]. Chinese Hepatolgy, 2019, 24(11): 1234-1236.
[10]	Li Qing, Lai Rong-tao, Lu Jie, Li Zi-qiang, Xie Qing, Wang Hui, Guo Qing. The role of SUMO specific protease 3 in HBV replication [J]. Chinese Hepatolgy, 2019, 24(1): 31-34.
[11]	XIA Li-fang, GUAN Bo-wen, SHAN Yao-bin, LI Kun-peng, WANG Tong, GUO Tong-sheng. Investigation of occult hepatitis B virus infection [J]. Chinese Hepatolgy, 2018, 23(9): 775-777.
[12]	HUANG Shun-mei, WANG Jun-zhong, ZHANG Xiao-yong, LI Bao-lin, SONG Zhi-tao, ZHU Zhen-ni, ZHU Bin, WANG Bao-ju, LU Meng-ji, YANG Dong-liang. Effect of three immunosupressive drugs dexamethasone, cyclophosphamide and tacrolimus on HBV replication in vitro [J]. Chinese Hepatolgy, 2018, 23(5): 391-394.
[13]	CHEN Li-wen, YAN Lei, CAO Zhu-jun, DING Ye-zhou, LIU Ke-hui, LIN Lan-yi, LAI Rong-tao, XIE Qing, WANG Hui. The role of RIPK3-mediated necroptosis in patients with HBV-related acute-on-chronic liver failure [J]. Chinese Hepatolgy, 2018, 23(3): 214-216.
[14]	YANG Zhibin, CHEN Qing, SHEN Enrui, KUANG Chongshu, LI Guowei, HOU Wenfeng, MA Wanhong, MA Shiwu. An investigation on the access to drugs against hepatitis B virus in comprehensive hospitals, Yunnan province (2018) [J]. Chinese Hepatolgy, 2018, 23(11): 969-972.
[15]	GAO Hai-bing, WANG Xiang-mei, LIU Bao-rong, PAN Chen, FANG Jian-kai, LIN Sheng-long, MA Hua-xi, CHEN Xiu-min, LIN Ming-hua, ZHENG Rui-dan. Expression of Treg and Th17 in HBV-related acute-on-chronic liver failure patients and their correlations with the mutations in HBV pre-C/BCP region [J]. Chinese Hepatolgy, 2018, 23(1): 18-21.

Three methods to identify hepatitis B virus strains with C/D recombinant

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments