Revealing role of miRNA in the pathogenesis of liver fibrosis by integrated bioinformatics analysis

Abstract

Abstract: Objective To reveal the potential role of micro-ribonucleic acid (microRNA) in the pathogenesis of liver fibrosis.Methods MicroRNA microarray GSE19865 and GSE66278 were downloaded from gene expression omnibus (GEO) database. The data was analyzed with GEO2R. The microRNAs up-regulated in both microarrays were analyzed by TargetScan and microT-CDS for target gene prediction, and target genes predicted by both were further subjected to functional analysis. Results In GSE19865, 37 microRNAs were up-regulated, and none was down-regulated. While in GSE66278, 19 microRNAs were up-regulated and 18 microRNAs were down-regulated. The miR-802 was upregulated both in GSE19865 and GSE66278. A total of 138 candidate target genes were predicted by both TargetScan and microT-CDS. Functional analysis revealed that mainly function of these genes were participated in pathways and biological processes like mitochondrial biogenesis, transforming growth factor-beta signaling pathway, histone lysine methylation, Wnt/Ca²⁺ pathway, etc. Protein-protein interaction analysis identified YWHAE, PPP2CA, RHOA, FOS, PSMD2, CDK19, ATF2 and PAFAH1B1 as hub genes.Conclusion By integrated bioinformatics analysis, miR-802 is involved in the liver fibrosis. Mechanistically, miR-802 regulates biological processes including histone methylation by targeting genes like YWHAE, PPP2CA and RHOA.

Key words: Liver fibrosis, microRNA, Bioinformatics

HUANG Chong, ZHENG Ya-hui, ZHANG Ju-bo. Revealing role of miRNA in the pathogenesis of liver fibrosis by integrated bioinformatics analysis[J]. Chinese Hepatolgy, 2019, 24(12): 1381-1386.

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