Chinese Hepatolgy ›› 2019, Vol. 24 ›› Issue (3): 234-238.

• Original Articles • Previous Articles     Next Articles

The analysis on association between IL28B single gene polymorphisms and the risk of intrauterine infection of HBV in HBsAg positive pregnant women

LI Rong, ZHANG Hang, HUANG Xi-yao, DU Dan   

  1. Department of Obstetrics and Gynaecology, Mianyang Third People′s Hospital (Sichuan Mental Health Center), Sichuan 6210000, China
  • Received:2018-09-19 Published:2020-03-28

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Abstract: Objective To investigate the association between interleukin 28B (IL28B) single gene polymorphisms (SNPs) and the risk of intrauterine infection of hepatitis B virus (HBV) in hepatitis B surface antigen (HBsAg) positive pregnant women.Methods Single fetus full-term HBsAg positive pregnant women were selected as the research objects, and were divided into infection group (658 cases) and non-infection group (1342 cases) according to whether HBV intrauterine infection occurred or not, and 542 healthy pregnant women were enrolled as the control group. Tetra-primer amplification refractory mutation system-polymerase chain reaction was used to detect the genotype of IL28B rs8099917 locus, the levels of serum interleukin-17 (IL-17), interleukin-21 (IL-21), interleukin-4 (IL-4), transforming growth factor-β1 (TGF-β1) and interferon-γ (IFN-γ) of patients with various genotypes of IL28B rs8099917 locus were measured and compared by enzyme-linked immunosorbent assay, and logistic regression was used to analyze the relationship between IL28B rs8099917 genotype with risk of HBV intrauterine infection. Results There were wild-type homozygous TT genotype, mutant heterozygous TG genotype and mutant homozygous GG genotype of IL28B rs8099917 locus, and the distribution of IL28B genotypes in each group followed Hardy-Weinberg equilibrium law. In infection group, the frequency of G allele was significantly higher than that of T allele (P<0.05), but there was no significant difference in non-infected group and control group (P>0.05). The levels of serum IL-17, IL-21, TGF-β1 and IL-4 in infection group were significantly higher than those in non-infected group and control group (P<0.05). In infection group, the levels in individuals with TG genotype were significantly higher than those with TT genotype (P<0.05), but significantly lower than those with GG genotype (P<0.05), while IFN-γ showed the opposite trend (P<0.05). However, in non-infection and control group, the differences were not significant (P>0.05).Conclusion IL28B SNPs of HBsAg positive pregnant women is related to HBV intrauterine infection, and GG genotype is a risk factor of HBV intrauterine infection.

Key words: Hepatitis B surface antigen, Interleukin 28B, Genetic polymorphism, Hepatitis B virus, Intrauterine infection