Effects of miR-509-3p and XIAP expression on proliferation and invasion of hepatocarcinoma cells

Abstract

Abstract: Objective To investigate the influence of the expression of microRNA-509-3p (miR-509-3p) and X-linked inhibitor of apoptosis protein (XIAP) on the proliferation and invasion of hepatocarcinoma cells, and to explore the mechanism.Methods Real-time polymerase chain reaction was used to detect the expression of miR-509-3p and XIAP in 107 liver cancer patients’ cancer tissues and paracancerous tissues. Cell proliferation assay and Transwell chamber invasion assay were used to observe the proliferation and invasion of HepG2 cells which were transfected with miR-509-3p mimics, miR-509-3p inhibitors, small interfering RNA silencing XIAP, respectively. Results The relative expression levels of miR-509-3p and XIAP in liver cancer tissues were 0.415±0.098 and 1.657±0.147, respectively, in paracancerous tissues were 1.127±0.126 and 0.425±0.113, respectively. The relative expression of miR-509-3p in liver cancer tissues was significantly lower than that in paracancerous tissues (t=3.257, P=0.002), while the relative expression of XIAP was significantly higher (t=4.201, P=0.000). There was a negative correlation between miR-509-3p and XIAP mRNA expression in liver cancer tissues (r=0.218, P=0.046). Compared with the control group, the proliferation rate of HepG2 cells was significantly lower at 48, 72, 96 and 120 hours of transfection with miR-509-3p analogs, and the difference was statistically significant (P<0.05). The proliferation rate of HepG2 cells was significantly higher after 48 hours of transfection with miR-509-3p inhibitors (P<0.05). Compared with the control group, the number of invasive HepG2 cells at 24 hours of transfection with miR-509-3p analogs was lower (96.32±0.52, 51.47±0.45, t=2.263, P= 0.021), the difference was statistically significant. Compared with the control group, the number of invasive HepG2 cell at 24 hours of transfection with miR-509-3p inhibitor was higher (94.65±0.42, 120.14±0.45, t=2.463, P=0.013), the difference was statistically significant.Conclusion The expression of miR-509-3p and XIAP are related to the proliferation and invasion of hepatocarcinoma cells. MiR-509-3p may regulate the proliferation and invasion of hepatocarcinoma cells by affecting the expression of XIAP.

Key words: MicroRNA-509-3p, X-linked inhibitor of apoptosis protein, Hepatocarcinoma cells, Proliferation and invasion

OUYANG Kao-bin, YUAN Xia, HE Yin. Effects of miR-509-3p and XIAP expression on proliferation and invasion of hepatocarcinoma cells[J]. Chinese Hepatolgy, 2019, 24(3): 243-247.

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