Abstract: Objective To explore the relationship between dysregulation of T and B cells in decidual tissue of intrahepatic cholestasis of pregnancy (ICP) during pregnancy and the degree of the disease.Methods One hundred and sixteen ICP pregnant women and 110 healthy physical examination pregnant women in our hospital between October 2017 and October 2019, and detection of T cell subtype and B cell content in decidual tissue by flow cytometry. The percentages of T and B cells in decidual mononuclear cells in ICP patients with different degrees of illness were analyzed, and the percentage of T and B cells in the decidual mononuclear cells in the decidual mononuclear cells was evaluated by ROC to evaluate the diagnostic efficacy of ICP.Results The percentages of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in the decidual tissue of ICP group accounted for (46.3±4.0)%, (19.2±4.2)%, (6.4±2.0)% and (1.2±0.2)%, significantly lower than the control group [respectively (54.0±3.0)%, (25.6±5.0)%, (14.5±3.2)% and (3.2±0.6)%, P<0.05].Among 116 patients with ICP, 86 were mild (74.1%) and 30 were severe (25.9%).The percentage of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in the decidual mononuclear cells of mild ICP was (47.8±4.3)%, (20.4±4.0)%, (6.6±1.3)% and (1.3±0.5)%, significantly higher than those with severe ICP [respectively (42.0±2.8)%, (15.6±4.5)%, (6.0±1.4)% and (0.9±0.3)%, P<0.05]. After ROC curve treatment, the results showed that the percentages of CD3⁺, CD3⁺CD4⁺, CD4⁺CD25⁺ and CD19⁺ in decidual mononucleocytes had certain diagnostic value for ICP, and the areas under the curve were 0.777, 0.744, 0.823 and 0.870, respectively.Conclusion The imbalance of T and B cells in decidua may be involved in the pathogenesis of ICP, and it is closely related to the severity of the disease.

Key words: Intrahepatic cholestasis of pregnancy, Decidual tissue, T cells, B cells