A preliminary study on the molecular mechanism of microRNA-27a inhibiting lipid metabolism of hepatic stellate cells

Abstract

Abstract: Objective To investigate the molecular mechanism of microRNA27a inhibiting the lipid metabolism of hepatic stellate cell. Methods LX2 cells were stimulated by palmitic acid (PA), the expression level of miR-27a was tested by PCR; After Overexpression of miR-27a, lipid deposition of LX2 cells was tested by oil red staining.The mRNA expression of FAS and SCD1 was verified by qPCR. C57BL/6 mice were randomly divided into two group: high-fatty diet with CCL₄ group(HFD+CCL₄) and low fatty diet with CCL₄ (LFD+CCL₄). The pathological changes of liver tissues in mice were detected by hematoxylin eosin (HE) and Sirius red staining. Besides, qPCR was applied to detect the relative expression of microRNA27a and the mRNA expression of FAS and SCD1 in different group. Results Comparing with the control group,the expression of miR27a in LX2 cell in experimental group decreased after stimulated by PA; In the mouse model ,the expression of miR27a in HFD with CCL₄ group also decreased, compared with LFD with CCL₄ (P<0.05).Compared with blank control group, lipid deposition in LX2 cells of overexpressing miR-27 a was significantly reduced as well as the mRNA expression of FAS and SCD1, however, the expression of FAS and SCD1 in HFD with CCL₄ group were increased (P<0.05). Conclusion MiR-27a can inhibit the lipid synthesis of hepatic stellate cells, reduce liver lipid deposition and delay the development of liver fatty change by inhibiting the expression of FAS and SCD1.This provides a new thought for the treatment of nonalchoholic fatty liver disease.

Key words: microRNA, FAS, SCD1, Nonalchoholic fatty liver disease

XU Zi-xin, LUO Xin, LUO Sheng-zheng, XU Ming-yi, LIN Ren-kun. A preliminary study on the molecular mechanism of microRNA-27a inhibiting lipid metabolism of hepatic stellate cells[J]. Chinese Hepatolgy, 2020, 25(9): 994-997.

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