Abstract: Objective The clinical features and prognostic data of non-hepatitis B and non- hepatitis C related hepatocellular carcinoma (NBNC-HCC) were retrospectively analyzed in order to provide references for the treatment strategies on NBNC-HCC patients.Methods A total of 94 patients (69 males and 25 females) with HCC were collected from January 2010 to June 2017, with an average age of 65.3±9.6 years. Twenty-six patients that were negative in serum HBsAg and HCV antibody (Ab) were grouped in NBNC-HCC group, including 19 males and 7 females. Sixty-eight patients that were positive in serum HBsAg or HCV Ab were grouped in non-NBNC-HCC group, with 50 males and 18 females. Multivariate Logistic regression analysis was used to explore the influencing factors of NBNC-HCC. Results The clinical data of NBNC-HCC group and non-NBNC-HCC group were compared. The average age of patients in NBNC-HCC group was (69.7±12.3) years old, while that in non-NBNC-HCC group was (62.4±8.3) years old. The difference was statistically significant (P<0.05). The alpha-fetoprotein (AFP) level in the patients of NBNC-HCC group 90.5 (65.8, 234.0) ng/mL were significant lower than that of the non-NBNC-HCC group 219. 5(95.0, 318.3) ng/mL(P<0.05). The ALT level in NBNC-HCC group (26.6±14.7) U/L were also different with that of the non-NBNC-HCC group (38.5±1.7) U/L (P<0.05). In NBNC-HCC group, 23 patients (88.5%) were Child-Pugh grade A, 3 patients (11.5%) were Child-Pugh grade B, while in NBNC-HCC group, 46 patients (67.6%) were Child-Pugh grade A, and 22 patients (32.4%) were Child-Pugh B, the difference was statistically significant (P<0.05). In NBNC-HCC group, there were 3 cases (11.5%) with vascular invasion and 23 cases (88.5%) without vascular invasion, while in non-NBNC-HCC group, there were 29 cases (42.6%) with vascular invasion and 39 cases (57.4%) without vascular invasion, the difference was statistically significant (P<0.05). In NBNC-HCC group, tumor boundary was clear in 17 cases (65.4%) and unclear in 9 cases (34.6%), while in non-NBNC-HCC group, tumor boundary was clear in 17 cases (25.0%) and unclear in 51 cases (75.0%), the difference was statistically significant (P<0.05). In NBNC-HCC group, there were 22 cases (84.6%) with tumor capsule and 4 cases (15.4%) without tumor capsule, while in non-NBNC-HCC group, there were 32 patients (47.1%) with tumor capsule and 36 patients (52.9%) without tumor capsule, the difference was statistically significant (P<0.05). Multivariate Logistic regression analysis showed that AFP, Child-Pugh grade, vascular invasion, tumor boundary and tumor capsule were independent risk factors for NBNC-HCC.Conclusion AFP, Child-Pugh grade, vascular invasion, tumor boundary and tumor capsule were independent risk factors for NBNC-HCC. The 3-year survival rate of NBNC-HCC patients was higher than that of non-NBNC-HCC patients.

Key words: non-hepatitis B and non- hepatitis C related hepatocellular carcinoma, α-fetoprotein, Child-Pugh classification, Logistic regression analysis