The HGF expression regulated by endoplasmic reticulum stress of hepatic stellate cells in the rats with acute liver failure

Abstract

Abstract: Objective To study the mechanism of endoplasmic reticulum stress (ERS) regulating the expression of hepatocyte growth factor (HGF) in hepatic stellate cells of rats with acute liver failure (ALF).Methods 60 clean SD rats were used as experimental animals. The 60 rats were divided into A, B and C groups by random number table, with 20 rats in each group. The three groups of rats were treated with D-galactosamine (D-GaIN) and lipopolysaccharide (LPS) to build ALF model. The rats in group A and C were treated with 0.6 mL·kg^-1 of tunicamycin one hour after modeling operation. The rats of group C were immediately intraperitoneally injected with 4-phenyl butyric acid (4-PBA) 500 mg·kg^-1 after completing the above operation. The rats in group B were negative control group without special intervention. There were 5 rats in each group, which were killed at 0 hour (T₁), 2 hours (T₂), 8 hours (T₃) and 12 hours after ALF modeling (T₄), the serum and liver tissue samples were collected, the serum HGF level in rats were measured. The expression of HGF in liver tissue of three groups of rats were detected by real-time-polymerase chain reaction (RT-PCR). Results The serum HGF of T₂, T₃ and T₄ were (63.9±7.0) ng/mL, (54.8±9.5) ng/mL and (42.0±6.7) ng/mL, respectively, which were significantly lower than group B [(82.3±10.6) ng/mL, (78.6±8.3) ng/mL and (76.2±9.0), P<0.05] and group C [(74.7±8.3) ng/mL, (70.4±10.2) ng/mL and (67.8±7.7) ng/mL, P<0.05]. There were significant difference in serum HGF level between group B and group C at T₂, T₃ and T₄ (P<0.05). The relative expression of HGF mRNA in T₂, T₃ and T₄ of group A were (2.9±0.5), (2.5±0.8) and (1.7±0.4), which were significantly lower than those of group B [(4.6±0.7), (4.7±0.9) and (4.4±0.7), P<0.05] and group C [(3.8±0.6),(3.5±0.7) and (3.1±0.8), P<0.05]. There were significant difference of HGF mRNA expression between group B and group C at T₂, T₃ and T₄ (P<0.05).Conclusion The endoplasmic reticulum stress (ERS) can regulate the expression of HGF in hepatic stellate cells of ALF rat and participate in the pathological process of ALF.

Key words: Endoplasmic reticulum stress, Acute liver failure, Hepatic stellate cells, HGF

LAN Yuan-qiang, WU Fa-sheng, HUANG Li-zhen, LIANG Min. The HGF expression regulated by endoplasmic reticulum stress of hepatic stellate cells in the rats with acute liver failure[J]. Chinese Hepatolgy, 2021, 26(5): 538-541.

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