miR-548a-5p regulates the apoptosis of hepatocellular carcinoma cell line HepG2 by targeting HMBOX1

Abstract

Abstract: Objective To investigate the potential mechanism of mir-548a-5p regulating the apoptosis of hepatocellular carcinoma (HCC) cell line HepG2.Methods MiR-548a-5p mimics and miR-548a-5p inhibitor were used to overexpress and knockdown miR-548a-5p and the apoptosis levels of HepG2 were detected. Potential substrates of miR-548a-5p were detected by miRDB online analysis and luciferase reporting system. The siRNA and PCDNA3.1 vector were used to overexpress substrates and the apoptosis level of HepG2 was detected.Results After overexpressing miR-548a-5p, the apoptosis level of HepG2 decreased, and the expression level of HMBOX1, a potential target of miR-548a-5p, decreased significantly. On the contrary, after knocking down miR-548a-5p, the apoptosis level of HepG2 increased, and the expression of HMBOX1 increased significantly (P<0.05). Luciferase reporter system showed that miR-548a-5p targeted the 3-terminal non-coding region of HMBOX1. After knocking down HMBOX1, the apoptosis level of HepG2 decreased significantly, and vice versa (P<0.05).Conclusion MiR-548a-5p inhibits the translation of HMBOX1 by targeting the 3-terminal non-coding region of HMBOX1 mRNA, thereby inhibiting the apoptosis of HepG2 of HCC cells.

Key words: MiR-548a-5p, HMBOX1, Hepatocellular carcinoma, HepG2, Apoptosis

YUAN Yi-ran, CHEN Long, ZHANG Zhen. miR-548a-5p regulates the apoptosis of hepatocellular carcinoma cell line HepG2 by targeting HMBOX1[J]. Chinese Hepatolgy, 2021, 26(8): 871-873.

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