Abstract: Objective To investigate the effect of liver indocyanine green fluorescent staining technique in the clinical teaching of residents in standardized training of liver surgery.Methods The residents who received standardized training in our department from January 2021 to December 2021 were randomly divided into two groups. The control group of 15 residents was taught by the traditional teachers’ explanation and the hepatectomy surgical training; the experimental group of 15 residents was given the traditional teaching method plus indocyanine green fluorescent staining of liver surgery training. Both groups took theoretical written and oral tests to check the effect of acquiring knowledge of liver anatomy and their satisfaction with teaching Methods.Results Residents in the experimental group had a deeper impression and understanding of the anatomical structure of the liver.Conclusion Indocyanine green fluorescence staining technology can make surgeons more intuitive, accurate and in-depth understanding of liver anatomy.

Key words: Indocyanine green, Fluorescent staining, Liver, Surgical training