Chinese Hepatolgy ›› 2022, Vol. 27 ›› Issue (12): 1296-1299.

• Liver Cancer • Previous Articles     Next Articles

The changes of histone acetylation modification in low invasive hepatocellular carcinoma cells

ZHANG Jian-dong1, LI Liang-he2, CHENG Hai-yu3, JIANG Dong-bin1, YANG Qin4, ZHAN Wei3*   

  1. 1. Clinical Medical School, Guizhou Medical University,Guiyang 550004,China;
    2. Department of Gastrointestinal, Affiliated Tumor Hospital of Guizhou Medical University,Guiyang 550004,China;
    3. Department of Anorectal,Affiliated Hospital of Guizhou Medical University, Guiyang 550004,China;
    4. Department of Pathophysiology,Guizhou Medical University, Guiyang 550004, China
  • Received:2022-01-08 Published:2023-01-30
  • Contact: ZHAN Wei,Email: 1564970707@qq.com

Abstract: Objective To investigate the changes of histone acetylation modification in normal hepatocyte line LO2 cells and low invasive hepatocellular carcinoma (HCC) line MHCC-97L cells.Methods The proliferation and apoptosis of LO2 and MHCC-97L cells were detected by real-time cell analysis (RTCA); the modification of acH3K14, acH3K18, acH3K27 in LO2 and MHCC-97L cells were analyzed by Western Blot.Results The results of RTCA showed that the proliferation rate of low invasive MHCC-97L cells was significantly higher than that of LO2 cells, of which the difference was statistically significant (P<0.05). There was little difference in apoptosis between the two cell lines under fluorescence microscope. The levels of acetylation modification of histone H3K14, H3K18, H3K27 in MHCC-97L cells were significantly lower than those in LO2 cells (P<0.05).Conclusion Compared to LO2 cells, the multiplication rate of MHCC-97L cells was significantly higher whereas the natural apoptosis rate was not different. The histone modification of acH3K14, acH3K18 and acH3K27 in MHCC-97L cells were decreased which might be associated with the hepatocarcinogenesis. Monitoring the levels of histone acetylation in hepatocytes may have a reference value for the clinical diagnosis of HCC and the upregulation of it may be a target for drug development against HCC.

Key words: Low invasive hepatocellular carcinoma cells, Proliferation, Apoptosis, Histone Acetylation