Abstract: Objective To study on the relationship between the levels of pregenomic RNA of hepatitis B virus (HBV pgRNA), DNA of hepatitis B virus (HBV DNA) and e antigen of hepatitis B (HBeAg) in patients with chronic hepatitis B (CHB).Methods A total of 72 CHB patients admitted from June 2018 to June 2021 were selected as the studied subjects, and the sample value/critical value (S/CO) was used as the result of HBeAg. Patients with 1<S/CO≤5 were included in the low-concentration group (n=16 cases). Patients with 5<S/CO≤10 were included in the medium-concentration group (n=35 cases). Patients with S/CO>10 were included in the high-concentration group (n=21 cases). The levels of HBV pgRNA, HBV DNA and HBeAg in three groups of patients were quantitatively detected, and the correlations between HBV pgRNA, HBV DNA and HBeAg was analyzed.Results The levels of HBV pgRNA in three groups of patients were (5.85±0.64) copies/mL, (7.12±0.38) copies/mL, (7.75±0.59) copies/mL, respectively. The HBV DNA levels were (5.34±0.49) copies/mL, (7.16±0.62) copies/mL, (9.35±0.54) copies/mL, respectively. The levels of HBV pgRNA and HBV DNA in these three groups were low concentration grouP<medium concentration grouP<high concentration group, P<0.05. Correlation analysis showed that HBV pgRNA was positively correlated with HBeAg (r=0.566, P=0.002), and HBV DNA was positively correlated with HBeAg (r=0.496, P=0.005).Conclusion With the increase of HBeAg level, HBV pgRNA and HBV DNA levels gradually increased. The serum HBV pgRNA and HBV DNA levels of CHB patients were positively correlated with HBeAg levels. Clinical diagnosis of HBV infection and replication and the condition of CHB patients could be more accurate by combining these three indicators.

Key words: Chronic hepatitis B, Hepatitis B virus pregenomic RNA, Hepatitis B viral DNA, Hepatitis B e antigen