The relationship between ANRIL and miR-122 expression in patients with occult hepatitis B viral infection

Abstract

Abstract: Objective To explore the application value of antisense noncoding RNA in cyclin kinase inhibitor 4 locus (ANRIL) and microRNA-122 (miR-122) in the differential diagnosis of occult hepatitis B virus (HBV) infection.Methods Twenty patients with occult hepatitis B admitted from February 2019 to January 2021 were selected as occult hepatitis B group. Eighty patients with chronic hepatitis B hospitalized at the same period of time were selected as chronic hepatitis B group. Eighty healthy people were enrolled as the healthy control group. Serum samples were collected from all groups of people. The serum levels of ANRIL and miR-122 were detected by real-time fluorescence quantitative PCR (qRT-PCR). The serum HBV DNA load was detected by PCR in combination with fluorescence probe in vitro. The levels of serum total bilirubin (TBil), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by automatic biochemical analyzer. The correlations between serum levels of ANRIL, miR-122, and TBIL, ALT, AST, and HBV DNA load in patients with occult hepatitis B were analyzed. The values of serum ANRIL and miR-122 in differential diagnosis of occult hepatitis B was analyzed. The influencing factors of occult hepatitis B were analyzed.Results The levels of TBIL, ALT, and AST in chronic hepatitis B group [(42.94±7.85) mol/L, (236.48±40.76) U/L, and (193.26±45.78) U/L, respectively] were higher than those in occult hepatitis B group [(25.64±6.35) mol/L, (32.74±7.15) U/L, and (29.36±7.24) U/L, respectively] and healthy control group [(22.46±4.68) mol/L, (26.95±7.03) U/L, and (19.46±6.13)U/L, respectively]. HBV DNA load in chronic hepatitis B group was higher than that in occult hepatitis B group [(6 243.58±1 006.74) vs (3.95±1.09)]. The miR-122 level in chronic hepatitis B group (0.43±0.12) was lower than that in occult hepatitis B group (0.69±0.21) and healthy control group (1.02±0.23) (P<0.05). The serum ANRIL level of occult hepatitis B group (1.53±0.37) was higher than that of healthy control group (1.01±0.21), and the miR-122 level was lower than that of healthy control group (P<0.05). Serum ANRIL was negatively correlated with miR-122 in patients with occult hepatitis B (r=-0.597, P<0.05), and ANRIL (miR-122) was positively (negatively) correlated with TBIL, ALT, AST and HBV DNA load (P<0.05). The area under the curve (AUC) of serum ANRIL, miR-122 and their combination in the differential diagnosis of occult hepatitis B was 0.901, 0.887 and 0.941, respectively; the specificity was 91.2%, 73.8% and 96.3%, respectively; and the sensitivity was 85.0%, 95.0% and 90.0%, respectively. ANRIL was an independent risk factor for occult hepatitis B (P<0.05), while miR-122 was a protective factor for occult hepatitis B (P<0.05).Conclusion The expression level of ANRIL is increased and the expression level of miR-122 is decreased in patients with occult hepatitis B. Both of them have good specificity and sensitivity in the differential diagnosis of occult hepatitis B.

Key words: occult hepatitis B, chronic hepatitis B, antisense noncoding RNA in cyclin kinase inhibitor 4 locus, microRNA-122, diagnosis

LI Xiang, WANG Yan-bai, WANG Yin-feng, HUANG Xue-lan. The relationship between ANRIL and miR-122 expression in patients with occult hepatitis B viral infection[J]. Chinese Hepatolgy, 2022, 27(3): 305-310.

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