The changes and correlation between oral and intestinal microflora in patients with liver cirrhosis

Abstract

Abstract: Objective To compare the differences between oral and intestinal microflora in patients with liver cirrhosis by 16S rRNA gene sequencing, and to analyze the association between them.Methods A total of 19 patients clinically diagnosed as liver cirrhosis were selected as the cirrhosis group (HS group), and 19 healthy volunteers were selected during the same period of time as the healthy group (HC). Fecal and saliva samples were collected from both groups of people. DNAs were extracted and 16S rRNA V3-V4 region gene sequencing were performed on the collected samples. The obtained high-quality data was compared with the Greengene database. The differences of oral and intestinal microflora between these two groups were further analyzed.Results Venn diagram showed that although there were some differences between oral and intestinal species, there also existed same species. At the phylum level, the abundance of Proteobacteria in HS group was significantly higher, whereas the abundance of Bacteroidetes was lower than that in HC group (all P<0.05). At the genus level, the abundance of Lachnospira in HS group was significantly lower, whereas the abundance of Escherichia and Veillonella were significantly higher than that in HC group (all P<0.05); As for the oral microflora at the phylum level, the abundance of Firmicutes in HS group was significantly higher, whereas the abundance of Proteobacteria was significantly lower than that in HC group (all P<0.05). At the genus level, the abundance of Veillonella and Leptotrichia was significantly higher, whereas the abundance of Haemophilus was significantly lower than that of HC group (all P<0.05). Alpha diversity analysis showed that the diversity and richness of oral and intestinal flora were decreased in patients with cirrhosis. Beta diversity results showed that there was significant difference between oral and intestinal microflora, and the difference between individuals of intestinal microflora was greater than that of oral microflora.Conclusion The oral and intestinal microflora composition of liver cirrhotic patients is very different, although there exists some common species. There was a significant increase in opportunistic bacteria and a significant decrease in beneficial bacteria in the oral and intestinal microflora of cirrhotic patients.

Key words: Liver cirrhosis, Oral microflora, Intestinal microflora, 16S rRNA

REN Hai-xia, YAN Hua-nan, NIU Shu-li, LI Bo, AN Kai, GUO Yong-ze, ZHANG Hong-wei. The changes and correlation between oral and intestinal microflora in patients with liver cirrhosis[J]. Chinese Hepatolgy, 2022, 27(3): 330-333.

References

[1] Bhat M, Arendt BM, Bhat V,et al. Implication of the intestinal microbiome in complications of cirrhosis. World J Hepatol, 2016, 8:1128-1136.
[2] 中华医学会肝病学分会. 肝硬化诊治指南. 中华肝脏病杂志, 2019:846-865.
[3] Baba Y, Iwatsuki M, Yoshida N, et al. Review of the gut microbiome and esophageal cancer: pathogenesis and potential clinical implications. Ann Gastroenterol Surg, 2017, 1:99-104.
[4] Komiya Y, Shimomura Y, Higurashi T, et al. Patients with colorectal cancer have identical strains of Fusobacterium nucleatum in their colorectal cancer and oral cavity. Gut, 2019, 68:1335-1337.
[5] Fan X, Alekseyenko AV, Wu J, et al. Human oral microbiome and prospective risk for pancreatic cancer: a population-based nested case-control study. Gut, 2018, 67:120-127.
[6] Kang Y, Cai Y. Gut microbiota and hepatitis-B-virus-induced chronic liver disease: implications for faecal microbiota transplantation therapy. J Hosp Infect, 2017, 96: 342-348.
[7] Grønkjær LL, Holmstrup P, Schou S, et al. Presence and consequence of tooth periapical radiolucency in patients with cirrhosis. Hepat Med, 2016, 8:97-103.
[8] Qin N, Yang F, Li A, et al. Alterations of the human gut microbiome in liver cirrhosis. Nature, 2014, 513:59-64.
[9] Iwauchi M, Horigome A, Ishikawa K, et al. Relationship between oral and gut microbiota in elderly people. Immun Inflamm Dis, 2019, 7:229-236.
[10] Bajaj JS, Torre A, Rojas ML, et al. Cognition and hospitalizations are linked with salivary and faecal microbiota in cirrhosis cohorts from the USA and Mexico. Liver Int, 2020, 40:1395-1407.
[11] Wang J, Tang H, Zhang C, et al. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. ISME J, 2015, 9:1-15.
[12] Zhang X, Shen D, Fang Z, et al. Human gut microbiota changes reveal the progression of glucose intolerance. PLoS One, 2013, 8:e71108.
[13] Walujkar SA, Dhotre DP, Marathe NP, et al. Characterization of bacterial community shift in human Ulcerative Colitis patients revealed by Illumina based 16S rRNA gene amplicon sequencing. Gut Pathog, 2014, 6:22.
[14] Thurnheer T, Belibasakis GN. Streptococcus oralis maintains homeostasis in oral biofilms by antagonizing the cariogenic pathogen Streptococcus mutans. Mol Oral Microbiol, 2018, 33:234-239.
[15] Ismail Y, Mahendran V, Octavia S, et al. Investigation of the enteric pathogenic potential of oral Campylobacter concisus strains isolated from patients with inflammatory bowel disease. PLoS One, 2012, 7:e38217.
[16] Strauss J, Kaplan GG, Beck PL, et al. Invasive potential of gut mucosa-derived Fusobacterium nucleatum positively correlates with IBD status of the host. Inflamm Bowel Dis, 2011, 17:1971-1978.
[17] 周慧芳, 万江丽. 肝硬化患者治疗前后肠道菌群的分析比较. 中国继续医学教育, 2018, 23:116-118.