Differentiation ratio of peripheral blood T cells in patients with primary biliary cirrhosis complicated with systemic lupus erythematosus and its clinical significance

Abstract

Abstract: Objective To investigate the differentiation ratio of peripheral blood T cells in patients with primary biliary cirrhosis (PBC) complicated with systemic lupus erythematosus (SLE) and its clinical significance.Methods Sixty patients with PBC complicated with SLE treated in our hospital from January 2018 to January 2021 were selected as the case group. In addition, healthy people who came to our hospital for physical examination in the same period were selected as the control group. The expression of T lymphocyte subsets in peripheral blood, mRNA expression of related inflammatory cytokines and secretion of Th1, Th2, Th17 cytokines in serum were compared between the two groups.Results The ratio of CD3+, CD4+, CD8+, CD16+, CD56+, Treg and CD4+/CD8+ in the case group were significantly lower than those in the control group [(67.84±9.56)% VS (74.85±8.95)%, (34.92±5.83)% VS (43.18±5.25)%, (18.39±1.58)% VS (21.83±1.24)%, (17.26±1.85)% VS (20.39±2.84)%, (3.15±1.23)% VS (4.82±1.42)%, (1.32±0.21)% VS (1.93±0.24)%, respectively, all P<0.05], and CD8+ was higher than that in the control group [(29.75±3.95)% VS (26.82±4.17)%, P<0.05]. The mRNA expressions of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-17A (IL-17A) and interleukin-22 (IL-22) in the control group were higher than those in the control group [(0.637±0.014) VS (0.575±0.023), (0.735±0.133) VS (0.641±0.037), (0.952±0.007) VS (0.631±0.005), (0.634±0.025) VS (0.593±0.036), (0.753±0.038) VS (0.522±0.023), (0.722±0.026) VS (0.512±0.041), (0.683±0.047) VS (0.573±0.045), (0.933±0.019) VS (0.566±0.012), respectively], the difference was statistically significant (all P<0.05). The secretion levels of Th1 (IFN-γ, TNF-α, IL-2), Th2 (IL-4, IL-6, IL-10) and Th17 (IL-17A, IL-22) cytokines in the case group were significantly higher than those in the control group (P<0.05).Conclusion There are significant differences in peripheral blood T cell subsets between patients with PBC complicated with SLE and healthy subjects. There are different degrees of abnormal cellular immune regulation and immune dysfunction.

Key words: Primary biliary cirrhosis, Systemic lupus erythematosus, Peripheral blood T cells, Flow cytometry, Cirrhosis

ZHOU Yan-yan. Differentiation ratio of peripheral blood T cells in patients with primary biliary cirrhosis complicated with systemic lupus erythematosus and its clinical significance[J]. Chinese Hepatolgy, 2022, 27(6): 698-702.

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