Abstract: Objective To investigate the current situation on the nucleic acid detection of hepatitis B virus (HBV) infection in voluntory unpaid blood donors in Baoding area, and to analyze the influencing factors.Methods A total of 207,000 donors with hepatitis B surface antigen (HBsAg) rapid test in our blood center from January 2019 to December 2020 were enrolled. Enzyme-linked immunoassay (ELISA) and viral nucleic acid detection technology (NAT) were performed in all cases. The clinical characteristics and influence factors of HBsAg-negative blood donors with HBV infection were compared and analyzed.Results The ELISA results of 207000 qualified samples showed that HBsAg positive in 949 cases, and the HBV DNA positive rate of HBsAg-negative cases was 0.050% (103/206051). The result of HBV DNA quantitative test in 103 HBsAg(-)/HBV DNA(+) blood donors showed that low viral load was the main reason, and the main serological model was hepatitis B surface antibody (anti-HBs) (+)/hepatitis B e antibody (antibody-HBe) (-)/hepatitis B core antibody (anti-HBc) (-). There was no significant difference in HBV infection rate among all blood donors with negative HBsAg screening at different times (P>0.05). There was also no significant difference in HBV infection rate between male and female (P>0.05). The HBV positive rate of blood donors with a history of intravenous drug use, unsafe sex, and blood transfusion was higher than those without the above-mentioned situation. (P<0.05); Logistic regression model analysis showed that the history of intravenous drug use, unsafe sex, and history of blood transfusion were influencing factors of HBV infection (P<0.05).Conclusion There is a certain rate of voluntary blood donors with HBV infection, and most of carriers with low viral load. Screening of risk factor should be further strengthened on the basis of ELISA screening to reduce the risk of HBV transmission.

Key words: Blood station, Nucleic acid detection, Hepatitis B virus infection, Logistic regression analysis