Beclin 1-mediated inhibitory effect of NS5ATP9 on starving-induced apoptosis in HepG2 cells

Abstract

Abstract: Objective To investigate Beclin 1-mediated inhibitory effect of NS5ATP9 on starving-induced apoptosis in hepatoblastoma cell line HepG2 cells.Methods HepG2 cells were serum starved and collected at different time points. The expression changes of NS5ATP9, Beclin1 and Bax proteins were detected by Western blot. In addition, HepG2 cells were transfected with NS5ATP9 siRNA or its overexpression vector for 48h. The cells were then serum starved for 24 h and collected for detection of bax by Western blot. Moreover, after knockdown of Beclin 1 by siRNA or overexpression of NS5ATP9 by transfected vector, the cells were serum starved for 24 h and collected for the detection of caspase-3/7 activity by chemiluminescence method. The expression of NS5ATP9, Beclin1 and Bax proteins were detected by Western blot.Results (1) the protein expressions of NS5ATP9 and bax in HepG2 cells were increased after 0, 6, 12, 18 and 24 h of serum starvation. The bax protein expression was increased After siRNA knockdown of NS5ATP9 expression. (2) After serum starvation for 24 h, the activity of Caspase-3/7 in HepG2 cells was increased in the Beclin 1 expression-inhibited cells (P=0.001), this was accompanied by a decreased effect of NS5ATP9 on inhibiting caspase-3/7 activity (P=0.000), and a weakened negative regulatory effect of NS5ATP9 on bax.Conclusion Beclin 1 partly mediated the inhibitory effect of NS5ATP9 on starving-induced HepG2 cell apoptosis through down-regulation of bax expression.

Key words: NS5ATP9, Starvation, Apoptosis, Beclin 1, Bax

QUAN min, XING Hui-chun. Beclin 1-mediated inhibitory effect of NS5ATP9 on starving-induced apoptosis in HepG2 cells[J]. Chinese Hepatolgy, 2022, 27(7): 782-784.

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