Abstract: Objective To investigate the expression of actin binding protein (ANLN), Cytochromes P450 (CYPs) subfamily members CYP2C9 and hyaluronic acid mediated movement factor receptor (HMMR) in hepatitis B virus-associated hepatocellular carcinoma (HBV-HCC) tissues and the effects on the prognosis of HCC patients. Methods Samples of 25 surgically resected HCC patients were collected, and immunohistochemistry was used to analyze the differences in the protein expression levels of ANLN, CYP2C9 and HMMR in tumor tissues and adjacent tissues, as well as the effects on postoperative recurrence and long-term survival of HCC patients were evaluated by log-rank test. Results The Immunoreactivity scoring system (IRS) of ANLN and CYP2C9 in tumor tissues of HCC patients were lower than those in adjacent tissues (ANLN:2.4±2.5 vs.3.7±2.7, t=2.18, P=0.039;CYP2C9:4.2±2.8 vs. 8.6±1.4, t=6.21, P<0.001), but the IRS of HMMR in tumor tissues was higher than that in adjacent tissues (8.0 (4.0, 8.0) vs. 4.0 (2.5, 4.0), Z=3.94, P<0.001). However, different protein expression levels of ANLN, CYP2C9 and HMMR had no significant difference in postoperative recurrence time and 5-year survival rate of HCC patients(Disease-free survival high vs. low:ANLN 38.6m vs.33.3 m, CYP2C9 36.5 m vs.35.5 m, HMMR 33.1 m vs.41.3 m;5-year overall survival high vs. low:ANLN 56.3 m vs.51.1 m, CYP2C9 55.1 m vs.51.5 m, HMMR 53.0 m vs.55.2 m;P>0.05). Multivariate Cox regression analysis showed that the influence of BCLC and family history on postoperative recurrence time was statistically different (HR=2.435, P=0.025;HR=0.165, P=0.009). AFP and family history were independently associated with 5-year survival rate in HCC patients (HR=229.488, P=0.041;HR=0.054, P=0.014). Conclusion The protein expression levels of ANLN, CYP2C9 and HMMR were different in HCC tumor and adjacent tissues. It suggested that they could be used as potential markers for the diagnosis of HCC.

Key words: Hepatocellular carcinoma, ANLN, CYP2C9, HMMR, prognosis