Evaluation value of serum miR-33a-5p and Sirt6 in liver fibrosis in infants with cholestasis

Abstract

Abstract: Objective To explore the evaluation value of serum microribonucleic acid 33a-5p (miR-33a-5p) and silent information regulatory factor 6 (Sirt6) in liver fibrosis in infants with cholestasis.Methods A total of 105 infants with cholestasis treated in our hospital from January 2021 to April 2023 were included as the study subjects (observation group). The degree of liver tissue fibrosis in infants was evaluated and grouped into a mild group of 41 cases and a moderate to severe group of 64 cases; 108 healthy infants who underwent routine examinations in our hospital were collected as the control group. Real-time fluorescence quantitative PCR (qRT-PCR) method was applied to detect serum miR-33a-5p level, enzyme-linked immunosorbent assay was applied to detect serum Sirt6 and four indicators: hyaluronic acid (HA), laminin (LN), type IV collagen (Ⅳ-C), and type III procollagen (PC-Ⅲ) levels in liver fibrosis; Pearson correlation analysis was applied to analyze the correlation between serum miR-33a-5p, Sirt6 levels and liver fibrosis indicators in infants with cholestasis; multivariate logistic regression analysis? was applied to analyze and screen the influencing factors of worsening liver fibrosis in infants with cholestasis; receiver operating characteristic curve (ROC curve) was applied to analyze the evaluation value of serum miR-33a-5p and Sirt6 levels for liver fibrosis in infants with cholestasis.Results The serum levels of miR-33a-5p (2.04±0.38), HA (218.95±49.47) ng/mL, LN (130.46±31.05) ng/mL, Ⅳ-C (147.36±40.51) ng/mL, PC-Ⅲ (547.18±92.75) ng/mL in the observation group were higher than those in the control group (1.01±0.24), (80.14±16.31) ng/mL, (17.64±4.98) ng/mL, (75.16±12.94) ng/mL, (140.43±38.22) ng/mL (P<0.05), while the serum Sirt6 (6.59±1.42) pg/mL level was lower than that in the control group (13.84±3.12) pg/mL (P<0.05). The serum miR-33a-5p level in infants with cholestasis was positively correlated with HA, LN, Ⅳ-C, and PC-Ⅲ (r=0.524, 0.508, 0.496, 0.501, P<0.05), while Sirt6 level was negatively correlated with HA, LN, Ⅳ-C, and PC-Ⅲ (r=-0.497, -0.516, -0.529, -0.514, P<0.05). The serum levels of miR-33a-5p, HA, LN, Ⅳ-C, PC-Ⅲ in infants in the moderate to severe group were higher than those in the mild group, while the serum Sirt6 level was lower than that in the mild group (P<0.05). MiR-33a-5p was identified as an independent risk factor for the worsening of liver fibrosis in infants with cholestasis (OR=2.948, P<0.05), Sirt6 was a protective factor for the worsening of liver fibrosis in infants with cholestasis (OR=0.795, P<0.05). The AUC of serum miR-33a-5p, Sirt6 levels and their combination for evaluating liver fibrosis in infants with cholestasis was 0.787, 0.825, and 0.907, respectively.Conclusion The combination of serum miR-33a-5p and Sirt6 levels showed high evaluation value for liver fibrosis in infants with cholestasis.

Key words: Cholestasis, Infants, Liver fibrosis, MiR-33a-5p, Silent information regulatory 6

CHANG Dong-zhe, GENG Xian-jie, ZHOU Liang, YANG Zi-jiu. Evaluation value of serum miR-33a-5p and Sirt6 in liver fibrosis in infants with cholestasis[J]. Chinese Hepatolgy, 2025, 30(3): 385-390.

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