Abstract: Objective To explore the relationship between GLUT-1, CDK4 and the clinical pathological characteristics and prognosis of patients with primary liver cancer. Methods Eighty-six patients with primary liver cancer admitted from December 2019 to June 2021 were included as the study subjects. All patients underwent surgical treatment and pathological examination, with a follow-up period of 3 years. The levels of GLUT-1 and CDK4 in cancer tissues and adjacent tissues were detected and compared. According to the prognosis, the patients were divided into a good prognosis group and a poor prognosis group. The relationship between the expression of GLUT-1 and CDK4 in primary liver cancer tissues and clinical pathological characteristics, as well as patient prognosis were analyzed. The patients were grouped based on their clinical stage and differentiation degree, and compare the levels of GLUT-1 and CDK4 in the two groups of patients. Kaplan Meier survival curves were used to evaluate the relationship between GLUT-1, CDK4, and prognosis of patients with primary liver cancer. Results The positive rates of GLUT-1 and CDK4 expression in cancer tissues of patients were 66.28% and 62.79%, respectively, which were significantly higher than those in adjacent tissues (22.09% and 18.60%, respectively) (P<0.05); There were significant differences (P<0.05) in lymphatic vessel metastasis and vascular invasion among liver cancer patients with positive expression of GLUT-1 and CDK4; The three-year cumulative survival rate of patients with GLUT-1 positive expression in liver cancer tissue was 42.11% (24/57), which was significantly lower than that of 79.31% (23/28) patients with GLUT-1 positive expression in liver cancer tissue (P<0.05); The three-year cumulative survival rate of patients with CDK4 positive expression in liver cancer tissue was 46.30% (25/54), which was significantly lower than that of 68.75% (22/32) patients with GLUT-1 negative expression in liver cancer tissue (P<0.05); Vascular invasion, and lymph node metastasis are independent risk factors affecting patient prognosis. Conclusion GLUT-1 and CDK4 are closely related to the clinical stage and differentiation degree of primary liver cancer patients, and can be used as important indicators to evaluate the patients' progression and poor prognosis.

Key words: Primary liver cancer, GLUT-1, CDK4, Pathological features, prognosis