Value of serum miR-524-5p and miR-411-5p expression in early diagnosis and clinical prognosis with liver cancer

Abstract

Abstract: Objective To explore the relationship between serum miR-524-5p and miR-411-5p expression in early diagnosis and clinical prognosis of patients with liver cancer (LC). Methods From October 2018 to October 2020, 96 patients with LC admitted to our hospital were collected as the LC group, and 96 healthy individuals from the same hospital were regarded as the control group. The expression levels of serum miR-524-5p and miR-411-5p were detected. The diagnostic efficacy of serum miR-524-5p and miR-411-5p levels in LC was analyzed. The relationship between the expression of serum miR-524-5p and miR-411-5p and prognosis in LC patients was analyzed. Further, the factors affecting the prognosis of LC patients were investigated. Results Compared with the control group, the expression level of miR-524-5p in LC group was significantly lower (0.76±0.19 vs. 1.01±0.21, t=8.625, P<0.001), and the expression level of miR-411-5p was also significantly lower (0.79±0.23 vs. 1.01±0.18, t=7.380, P<0.001). The area under the curve (AUC) for the combined diagnosis of LC using serum miR-524-5p and miR-411-5p was the highest at 0.850, which was better than the individual diagnosis of serum miR-524-5p and miR-411-5p (Z_{combination-miR-524-5p}=2.126, P=0.034, Z_{combination-miR-411-5p}=3.577, P<0.001). The expression level of miR-524-5p and miR-411-5p in LC patients was related to TNM staging, lymph node metastasis, depth of infiltration, and degree of differentiation (P<0.05). The 3-year survival rate of LC patients with high expression level of miR-524-5p and miR-411-5p was higher than that of patients with low expression of miR-524-5p and miR-411-5p (χ²=4.525, 5.596, P=0.033, 0.018). Multivariate Cox analysis showed that miR-524-5p and miR-411-5p were protective factors affecting the prognosis of LC patients (P<0.05), and TNM stage, lymph node metastasis, invasion depth and differentiation degree were risk factors affecting the prognosis of LC patients (P<0.05). Conclusion The expression levels of serum miR-524-5p and miR-411-5p are decreased in LC patients, and their expressions are related to clinicopathological features and prognosis. The combination of them has the best efficacy in diagnosing LC.

Key words: Liver cancer, MiR-524-5p, MiR-411-5p, Early diagnosis, Prognosis

CHEN Jie, TIAN Le, WU Ke, ZHANG Qian. Value of serum miR-524-5p and miR-411-5p expression in early diagnosis and clinical prognosis with liver cancer[J]. Chinese Hepatolgy, 2025, 30(9): 1238-1243.

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