The expression and clinical significance of serum lncRNA-H19 and FOXO1 levels in children with infantile cholestatic hepatopathy

Abstract

Abstract: Objective To investigate the expression and clinical significance of serum lncRNA-H19 and forkhead transcription factor O1 (FOXO1) levels in children with infantile cholestatic hepatopathy (ICH). Methods 100 children with ICH who received treatment at our institute from April 2017 to April 2022 were included as the study subjects (ICH group). According to the score of clinical symptoms and signs, they were further divided into mild group (<10 points, 79 cases) and moderate and severe group (≥10 points, 21 cases). Another 100 infants who developed jaundice in the same period without liver disease were included as control group. The serum levels of lncRNA-H19 and FOXO1 in children were measured by fluorescence quantitative real time PCR; logistic multiple factor regression was applied to analyze the influencing factors of patients with ICH; the serum levels of lncRNA-H19, FOXO1 and their combined diagnostic value for ICH were evaluated by the subject working characteristic (ROC) curve. Results Compared with the control group, the levels of lncRNA-H19 (1.39±0.43 vs. 1.00±0.33) in ICH group were obviously higher, the levels of FOXO1 (0.74±0.24 vs. 1.01±0.32) were obviously lower, and the difference were statistically significant (t=7.195, 6.750, P<0.05). Compared with the mild group, lncRNA-H19 (1.78±0.47 vs. 1.29±0.32) levels in the moderate and severe group were significantly higher , FOXO1 (0.48±0.13 vs. 0.81±0.22) levels were significantly lower (t=5.610, 6.561, P<0.05). Logistic analysis showed that lncRNA-H19 (OR: 1.715, 95%CI:1.212~2.426) were independent risk factors for ICH, and high levels of FOXO1 (OR: 0.593, 95%CI:0.374~0.940) were independent protective factors for ICH (P<0.05). ROC results showed that the AUC of the severity of the levels of lncRNA-H19 and FOXO1 in serum and their combination for ICH was 0.758, 0.750 and 0.829, respectively, the AUC of combined prediction was obviously higher than the two independent prediction (Z=1.589, 1.738, P<0.05). Conclusion The level of serum lncRNA-H19 is increased and the level of FOXO1 is decreased in children with ICH, they are closely related to the severity of ICH and are expected to become predictors of ICH.

Key words: Infantile cholestatic hepatopathy, LncRNA-H19, Forkhead transcription factor O1, Diagnosis

FANG Wen-zheng, LI Dao-xing, ZUO Li. The expression and clinical significance of serum lncRNA-H19 and FOXO1 levels in children with infantile cholestatic hepatopathy[J]. Chinese Hepatolgy, 2026, 31(4): 578-582.

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