Abstract: Objective To observes the incidence of alteration of intestinal flora and non-alcoholic fatty liver disease in obese children, and to analyze the effect of alteration of intestinal flora on the incidence of non-alcoholic fatty liver disease in obese children. Methods 541 obese children in the outpatient of hospital from March 2017 to June 2019 were selected as obese group, and 112 children who received regular physical examination and were confirmed to be non-obese and had no other serious complications during the same period were selected as control group. The general situations questionnaire was designed, and the general situations of the research subjects were asked and recorded. All children underwent intestinal flora examination. The incidence of alteration of intestinal flora and non-alcoholic fatty liver disease in obese group and control group were compared, and the relationship between alteration of intestinal flora and non-alcoholic fatty liver disease in obese children was analyzed. Results The body mass index (BMI) and waist circumference in obese group were [(29.17±1.11)kg/m², (81.21±5.54)cm], which were higher than those in control group (P<0.05); Compared with control group, the detected number of enteric bacilli and enterococcus colony in obese group was [(8.62±1.33), (7.49±1.11)], which was higher, and the detected number of other colonies was lower; The detection rate of alteration of intestinal flora and non-alcoholic fatty liver diseasewas (18.67%, 9.43%), which was high (P<0.05); The alteration of intestinal flora was positively correlated with the incidence of non-alcoholic fatty liver disease (r=0.673, P<0.05); The detection rate of non-alcoholic fatty liver disease in obese children with alteration of intestinal flora was (41.58%), which was higher than that in normal people (P<0.05); The alteration of intestinal flora was the risk factor for non-alcoholic fatty liver disease in obese children (OR=34.090, P<0.05). Conclusion Obese children are often associated with alteration of intestinal flora, which is closely related to the occurrence of non-alcoholic fatty liver disease. Alteration of intestinal flora may be the risk factor for the occurrence of non-alcoholic fatty liver disease in obese children, and it should be paid clinical attention to detect and intervene as soon as possible.

Key words: Non-alcoholic fatty liver disease, Children, Obese, Alteration of intestinal flora