Table of Content

31 May 2018, Volume 23 Issue 5

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Original Articles

Roles and limitations of two common non-invasive methods in diagnosis of liver fibrosis

CHEN Yang-yi, LV Jing, LIU Cheng-hai, ZHAO Zhi-min, CHEN Gao-feng

2018, 23(5):  381-384. 

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Objective To investigate the value and limitation of abdominal ultrasonography (AUS) and FibroTouch (FT) in the diagnosis of liver fibrosis for better clinical application. Methods Liver stiffness measurement and semi-quantitative AUS score were recorded. Diagnostic value of AUS and FT for liver fibrosis was assessed with receiver operating characteristic curve using liver biopsy as the gold standard. Moreover, cases misdiagnosed of cirrhosis by the two methods were comparatively analyzed. Results The areas under the curve of AUS and FT for apparent fibrosis (S≥2), progressive fibrosis (S≥3) and early cirrhosis (S=4) were 0.915, 0.873, 0.821 and 0.854, 0.802, 0.727, respectively. There were 87 cases (34%) in AUS group and 45 cases (27%) in FT group with biased diagnosis. Conclusion AUS and FT may serve as two reliable ultrasonic non-invasive methods for the diagnosis of liver fibrosis, which can be applied for observation of clinical efficacy. However, liver biopsy and non-invasive methods should be used under comprehensive analysis because of their disadvantages.

Correlation of recurrence time with lipid content and pseudocapsule MR characteristics in small hepatocellular carcinoma

LIU Chang-chun, WANG Wei-wei, REN Hong-wei, AN Wei-min, DONG Jing-hui, ZHANG Jian

2018, 23(5):  385-386. 

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Objective To investigate the correlation of recurrence time after surgical resection with lipid content and pseudocapsule magnetic resonance (MR) features in small hepatocellular carcinoma (HCC). Methods Patients receiving operation for small HCC in our hospital from February 2011 to November 2016 were followed up, who had undergone dynamic enhanced MR scan within 1 month before operation. Thirty-four patients with HCC recurrence or without HCC recurrence during 30-month follow-up were enrolled, who were divided into >30 months group, 10~30 months group and <10 months group. Double echo sequence was used to detect lipid content and the signal characteristics of pseudocapsule were observed in preoperative MR images. Statistical analysis was performed. Results Among the patients, 15 were with >30 months recurrence time, 12 were with 10~30 months recurrence time, and 7 were with <10 months recurrence time. In<10 months group, no lipids were detected in the lesions. However, in the other two groups, 10 cases had lipid content in lesions and 17 cases had no lipid content in lesions. In <10 months group, the pseudocapsules of lesions showed short, equal, long T1 and short, equal T2 signals. In 10~30 months group, the pseudocapsules of lesions could be characterized by short, equal, long T1 and T2 signals. Conclusion Lesions containing lipid and pseudocapsule showing long T2 signal in MR indicate longer disease-free survival time after surgical resection.

Treatment of transplanted hepatocarcinoma in mice with sequential injection of inactivated heterogeneic lymphocyte and autogeneic lymphocyte

WU Lin-lan, XU Bing, HUANG Su-qin, WEI Jian-wei, YANG Xiao-mei, ZHAO Zhi-ping, CHEN Yi, JIANG Xiao-zhi

2018, 23(5):  387-390. 

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Objective To investigate the anti-tumor effect of sequential injection of heterogeneic lymphocyte (HL) and autogeneic lymphocyte (AL). Methods Inactivated heterogeneic lymphocyte (IHL) was isolated from spleen of C57BL/6 mice and inactivated with mitomycin. Hepa1-6 cells were inoculated into liver tissue of CB6F1 mice. Fibrin glue (FG) was prepared with cryoprecipitate collected from mouse plasma using freeze-thaw method. The experimental treatment consisted of two stages. At first stage (13 days), tumor-bearing mice were injected with FG-IHL in liver as experiment group, and injected with FG-phosphate buffer saline (FG-PBS) in liver as control group. Immunological indices such as tumor cell killing rate of the spleen lymphocytes and number of lymphocytes, CD8⁺T and NK in the two groups were detected, respectively. At the later stage (8 days), FG-AL was prepared from randomly selected tumor-bearing mice in experiment and control group, which was injected into the liver of rest tumor-bearing mice in the same group, respectively. After 8-day treatment, tumor size and inhibition rate were compared between the two groups. Results The tumor cell killing rate of AL in experiment group was significantly higher than that in control group (25.7±4.81% vs 5.9±1.64,P<0.01). Numbers of spleen lymphocyte, CD8⁺T and NK cell in experiment group were significantly higher than those in control group, respectively (all P<0.05). After 2-stage treatment, mean volume of tumor in experiment group was significantly smaller than that in control group (1.15±0.25 cm³ vs. 1.91±0.37 cm³, P<0.01). Tumor inhibiting rate in experiment group was 39.8%. Conclusion The treatment of sequential injections with FG-IHL followed by the FG-AL in situ could suppress the growth of transplanted tumor in mice, which might become a promising biotherapy to treat cancers.

Effect of three immunosupressive drugs dexamethasone, cyclophosphamide and tacrolimus on HBV replication in vitro

HUANG Shun-mei, WANG Jun-zhong, ZHANG Xiao-yong, LI Bao-lin, SONG Zhi-tao, ZHU Zhen-ni, ZHU Bin, WANG Bao-ju, LU Meng-ji, YANG Dong-liang

2018, 23(5):  391-394. 

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Objective To investigate the effect of dexamethasone (DEX), cyclophosphamide (CYP) and tacrolimus (FK506) on DNA replication and protein synthesis of hepatitis B virus (HBV) in vitro. Methods HepG2.2.15 cell line was treated with different concentrations of DEX, CYP or FK506, and safe concentrations of the three immunosupressive drugs were identified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Levels of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in the cell culture supernatant were detected with electrochemiluminescence immunoassay. The intracellular HBV DNA level was evaluated with Southern blot. HBV expression and replication were assessed according to HBsAg, HBeAg and HBV DNA level. Results The safe concentration range of DEX, CYP and FK506 was 0～500 μg/mL, 0～1000 μg/mL and 0～10 μg/mL, respectively. Compared with control group, FK506 group showed no significant difference in the levels of HBsAg, HBeAg and HBV DNA. CYP group showed higher levels of HBsAg (P<0.05), while no significant difference in the levels of HBeAg and HBV DNA (P>0.05). DEX group showed lower levels of HBsAg and HBeAg (P<0.05), while no significant difference in HBV DNA (P>0.05). Conclusion In the safe concentration range, FK506 has no direct inhibitory or promoting effect on HBV DNA replication. CYP treatment could increase HBsAg expression, but have no effect on HBeAg expression and HBV DNA replication. DEX treatment could inhibit the expression of HBsAg or HBeAg, but has no inhibitory effect on HBV DNA replication.

Interaction between transcription factor Slug and KLF5 and its effect on the migration of hepatoma cells

YAN Yun-qing, WANG Zhi-liang

2018, 23(5):  395-399. 

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Objective To investigate the interaction between transcription factor Slug and Krüpple-like factor 5 (KLF5), and whether Slug acts as a downstream signaling factor of KLF5 and affect the migration ability of hepatocellular carcinoma (HCC) cells. Methods The interaction and co-localization between Slug and KLF5 were detected by immunoprecipitation and immunofluorescence assay. Slug overexpression plasmid was transfected into HCC cells and shRNA was used to inhibit the expression of Slug in HCC cells. Migration ability of HCC cells was observed. Results In HCC cells, the expression of transcription factor Slug was found altering with the change of KLF5, and their interaction and co-localization were at protein level. However, upregulation or downregulation of Slug expression in HCC cells did not affect the migration ability of HCC cells. Conclusion The transcription factor KLF5, which is known to inhibit the migration of HCC cells, is found to interact with another transcription factor Slug and has cell co-localization with Slug. However, transcription factor Slug is not a downstream signaling factor of KLF5 and cannot affect the migration ability of HCC cells.