Interaction between transcription factor Slug and KLF5 and its effect on the migration of hepatoma cells

Abstract

Abstract: Objective To investigate the interaction between transcription factor Slug and Krüpple-like factor 5 (KLF5), and whether Slug acts as a downstream signaling factor of KLF5 and affect the migration ability of hepatocellular carcinoma (HCC) cells. Methods The interaction and co-localization between Slug and KLF5 were detected by immunoprecipitation and immunofluorescence assay. Slug overexpression plasmid was transfected into HCC cells and shRNA was used to inhibit the expression of Slug in HCC cells. Migration ability of HCC cells was observed. Results In HCC cells, the expression of transcription factor Slug was found altering with the change of KLF5, and their interaction and co-localization were at protein level. However, upregulation or downregulation of Slug expression in HCC cells did not affect the migration ability of HCC cells. Conclusion The transcription factor KLF5, which is known to inhibit the migration of HCC cells, is found to interact with another transcription factor Slug and has cell co-localization with Slug. However, transcription factor Slug is not a downstream signaling factor of KLF5 and cannot affect the migration ability of HCC cells.

Key words: Transcription factor, KLF5, Slug, Hepatocellular carcinoma, Migration

YAN Yun-qing, WANG Zhi-liang. Interaction between transcription factor Slug and KLF5 and its effect on the migration of hepatoma cells[J]. Chinese Hepatolgy, 2018, 23(5): 395-399.

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