Table of Content

15 January 2017, Volume 22 Issue 1

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Original Articles

Assessment value of indocyanine green clearance test for preoperative liver reserve function of patients with primary liver cancer

HUANG Wen-qi, XU Jin-chao, MIN Feng, WU Wei-bing, FAN Rong-hua, ZHANG Li

2017, 22(1):  6. 

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Objective To investigate the indocyanine green clearance test for preoperative liver reserve function of patients with primary liver cancer.Methods Fifty healthy people and 71 patients were enrolled as control group and study group,respectively.Indocyanine green clearance rate in 15 minutes（ICGR15）,hepatic function,blood routine test and abdominal ultrasound were measured in all patients,and the Child-Turcotte-Pugh （CTP）scores were calculated for classification.All data were analyzed by student＇s t-test,one-way analysis of variance and linear correlation analysis with SPSS 17.0.Results The levels of ICGR15 in patients with primary liver cancer（18.7±4.3）were higher than those in controls（4.7±1.2）（t=–22.39,P<0.05）.Moreover,levels of ICGR15 were positively correlated with the CTP score（r=0.722,P<0.05）,but negatively correlated with hepatic blood flow（r=–0.889,P<0.05）and plasma clearance rate（r=–0.753,P<0.05）.Comparisons in levels of prothrombin time（PT）,international normalized ratio（INR）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,albumin（Alb）and total bilirubin（TBil）among patients with different levels of ICGR15 showed statistically differences（t=3.39,61.9,2.62,3.19,69.0,40.5;P<0.05）.Meanwhile,levels of ICGR15 was positively correlated with the levels of PT,INR,AST and TBil（r=0.665,0.527,0.316,0.721;P<0.05）,but negatively correlated with the levels of Alb（r=–0.507,P<0.05）.Furthermore,ICGR15 showed strongest correlation with the levels of PLT and TBil.Conclusion The indocyanine green clearance test could dynamically reflect liver reserve function,which is more accurately than CTP classification.

The polymorphism of UGT1A1 gene in patients with Gilbert＇s syndrome

SUN Mei, TAN Guo-lei, WANG Jianfang, WU Xu-ping

2017, 22(1):  7. 

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Objective To investigate the polymorphism of UDP glucuronosyltransferase family 1 member A1（UGT1A1）in 29 patients with Gilbert＇s syndrome and 22 healthy controls.Methods DNA isolated from peripheral blood and amplified by polymerase chain reaction（PCR）was subjected for sequencing UGT1A1 gene exons and enhancer regions.Results UGT1A1 gene variations were detected in enhancer region,TATA box region and the exon 1 region,respectively.Among 29 patients with Gilbert＇s syndrome,10 G/G homozygosis and 15 T/G heterozygosis were detected in enhancer region UGT1A1*60 3729（T>G）,15 TATA（7）TAA homozygosis and 2 TATA（6）/TATA（7）TAA heterozygosis in TATA box region,and 3 A/A homozygosis and 15 G/A heterozygosis in exon 1 region.In 20 healthy controls,2 G/G and 11 T/G mutations were detected in enhancer region UGT1A1*60 3729（T>G）,7 homozygous TATA（6）/TATA（7）TAA mutation in TATA box region,and 5 G/A mutations in exon 1 region.Conclusion UGT1A1 gene mutations in patients with Gilbert＇s syndrome is higher than that in healthy controls,which could only be used as a reference index for diagnosis of Gilbert＇s syndrome.

Exression and clinical sinificance of HBx and Beclin1 in heatocellular carcinoma

DUAN Chang-hu, LIU Xiaochen, DOU Fa-fu, DING Jian-long, DUAN Jian-feng, ZHAO Xi-rong, ZHOU Ya-dong, LI Guang-hua, YANG Fan

2017, 22(1):  8. 

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Objective To investigate the specific role of hepatitis B virus X protein （HBx）and Beclin1 protein（Beclin1）in the pathogenesis of hepatocellular carcinoma（HCC）and their correlations.Methods Carcinoma tissue and adjacent tissue from 64 patients pathologically diagnosed as HCC without any treatment before surgery were collected in our hospital from January 2014 to December 2015.The expressions of HBx and Beclin l in carcinoma tissue and adjacent tissue of all cases were detected by real-time polymerase chain reaction（PCR）,western blot and immunohistochemistry（IHC）,which were analyzed in detail combined with their clinical data.Results Compared to those in adjacent tissue,higher levels of HBx and Beclin1 were detected by IHC in 82.8%and 79.7%of HCC tissue,respectively.Meanwhile,both HBx and Beclin1 showed higher protein levels in HCC tissues than that in adjacent tissues by western blot,as well as m RNA levels（0.78±0.10 vs.0.11±0.01 and 0.83±0.15 vs.0.18±0.03）by real-time PCR,respectively.Conclusion HBx and Beclin1 are both overexpressed in HCC and might participate in the pathogenesis of HCC.Combined detection of HBx and Beclin1 might play an important role in prediction of occurrence and metastasis of HCC,which might be potential target genes for HCC treatment.

Correlative factors of glomerular filtration rate in chronic hepatitis B patients

GU Sheng-wang, ZHOU Shu, LIU huan, ZHAO Ya-ping, CHEN Chu-chu, ZHU Qian, LIU Chun-yan

2017, 22(1):  9. 

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Objective To investigate the correlations of glomerular filtration rate（GFR）with age,creatinine level and other factors in chronic hepatitis B patients.Methods GFR was detected in a total of 1060 patients,including 228 people as contrast group,563 people as chronic hepatitis（CH）group,98 people as early liver cirrhosis（ELC）group,41 people as hepatocellular carcinoma（HCC）group,and 130 people as decompensated liver cirrhosis（HLC）group.Chisquare test,independent sample t-test and bivariate Pearson correlation analysis were applied in the study by SPSS 19.0.Results In contrast group,CH group,ELC group,HCC group and HLC group,there were 38（16.8%）and 4（1.7%）,108（19.2%）and 3（0.5%）,26（26.5%）and 6（6.1%）,8（19.5%）and 0（0%）,and 43（33.1%）and 16（12.3%）patients with mildly and moderately decreased GFR,respectively.Among all those 832 patients with chronic hepatitis B,the correlation coefficients of age,creatinine,urea nitrogen and uric acid with GFR were–0.369,–0.597,–0.282 and–0.2,respectively.Conclusion GFR is more accurate in assessing renal function impairment,especially for early and mild renal dysfunction.Influence of nucleoside analogues on GFR in chronic hepatitis B patients should be paid more attention.Additionally,both GFR and blood phosphorus test play indispensable roles in clinical practice and cannot replace one another.

A preliminary study of between Bcl-2-modified bone marrow mesenchymal stem cell therapy in acute liver failure

YANG Peng-fei, XIE Jing-dong, YAN Bing-zhu, YANG Bao-shan

2017, 22(1):  10. 

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Objective To construct Bcl-2 gene-modified lentivirus,establish stable expression of Bcl-2 gene in bone marrow mesenchymal stem cell lines,and discuss the feasibility of bone marrow Bcl-2 gene-modified mesenchymal stem cells therapy in acute liver failure（ALF）to the problems of liver apoptosis and regeneration.Methods Bcl-2 gene fragment synthesized by polymerase chain reaction（PCR）was inserted into GV287 empty vector to be amplified and packaged in lentivirus.Bcl-2 lentivirus were transfected into rat bone marrow mesenchymal stem cells.Then bone marrow mesenchymal stem cells were transplanted into rats with acute liver injury,and survival rates and liver pathology were observed after transplantation.Results The purified bone marrow mesenchymal stem cells lines were obtained.Bcl-2 gene fragments were separated for construction of Bcl-2 gene lentivirus,which was transfected into bone marrow mesenchymal stem cells successfully.Through detecting by green fluorescent protein（GFP）fluorescence and western blot,transfected cells were in good condition with high expression of Bcl-2.Rat models were transplanted with bone marrow mesenchymal stem cells through tail vein,and PKH26 fluorescence-labeled bone marrow mesenchymal stem cells can be detected in damaged liver tissue by liver pathology frozen section after transplantation.Conclusion We preliminarily confirm that bone marrow mesenchymal stem cells can home to liver tissue in rats with acute liver injury with certain therapeutic effects.