白藜芦醇与熊去氧胆酸治疗妊娠期肝内胆汁淤积症的疗效比较

肝脏 ›› 2017, Vol. 22 ›› Issue (8): 685-689.

白藜芦醇与熊去氧胆酸治疗妊娠期肝内胆汁淤积症的疗效比较

廖娥,邵勇

400016 重庆医科大学附属第一医院妇产科

收稿日期:2017-04-12 出版日期:2017-08-15 发布日期:2020-06-16
通讯作者: 邵勇,Email: cqshaoyong@163.com
基金资助:
国家自然科学基金资助项目(81471473),重庆市卫计委医学高端后备人才培养项目(2015XMSB0001298)

Efficacy comparison between resveratrol and ursodeoxycholic acid in the treatment of intrahepatic cholestasis of pregnancy

LIAO E, SHAO Yong

The Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received:2017-04-12 Online:2017-08-15 Published:2020-06-16
Contact: Shao Yon, E-mail: cqshaoyong@163.com

摘要/Abstract

摘要： 目的比较白藜芦醇(RES)与熊去氧胆酸(UDCA)治疗妊娠期肝内胆汁淤积症(ICP)孕鼠效果,探讨RES治疗ICP的可行性及疗效。方法采用17α-乙炔雌二醇建立ICP模型,将孕13 d孕鼠70只随机分为对照组、ICP组及治疗组(LRES、MRES、HRES、UDCA、UDCA-MRES),测定用药前后TBA及ALT水平,统计各组死胎率,HE染色观察母鼠肝脏形态学改变,免疫组化及Western印迹测定肝内SIRT1及TNF-α水平。结果与ICP相比[胆汁酸水平(49.38±13.68) μmol/L,死胎率32.26%],LRES有明显降胆汁酸效果[(31.81±15.19) μmol/L,P<0.05],MRES降低死胎率效果明显(14.29%,P<0.05);UDCA-MRES联合用药存在协同降胆汁酸作用[(27.77±7.43) μmol/L,P<0.05],却比单独用药导致更高的死胎率(36.26%,P<0.05);RES能够明显增加SIRT1表达(P<0.05),降低TNF-α水平(P<0.05)。结论 RES与UDCA一样具有治疗ICP的可能,但其有效剂量仍需进一步探究。

关键词: 白藜芦醇, 熊去氧胆酸, 妊娠期肝内胆汁淤积症

Abstract: Objective To compare the efficacy between resveratrol (RES) and ursodeoxycholic acid (UDCA) in the treatment of intrahepatic cholestasis of pregnancy (ICP).Methods Seventy Sprague-Dawley (SD) rats on day 13 of pregnancy were induced ICP using 17-α ethinyl estradiol, and divided into control group, ICP group and 5 treatment groups [low-dosage RES (LRES) group, middle-dosage RES (MRES) group, high-dosage RES (HRES) group, UDCA group and UDCA combined with MRES (UDCA-MRSV) group]. Livers were collected for histological analysis using hematoxylin and eosin (HE) staining. Serum levels of total bile acid (TBA) and alanine aminotransferase (ALT) were measured before and after treatment in all 5 treatment groups. Stillbirth rate of each group was counted. Immunohistochemistry and western blot were applied for determining intrahepatic levels of silent information regulator 1 (SIRT1) and tumor necrosis factor-α (TNF-α).Results Compared with ICP group, LRES could obviously decrease TBA (31.81±15.19 umol/L, P<0.05), and MRES could obviously decrease the stillbirth rate (14.29%, P<0.05). UDCA-MRSV might synergistically reduce TBA level (27.77±7.43 umol/L, P<0.05), but with a higher rate of stillbirth (36.26%, P<0.05) than the single drug treatment. RES could enhance SIRT1 expression (P<0.05) and decrease TNF-α expression (P<0.05).Conclusion RES might be a potential option in treatment of ICP, of which effective dosage needs further investigation.

Key words: Resveratrol, Ursodeoxycholic acid, Intrahepatic cholestasis of pregnancy

廖娥,邵勇. 白藜芦醇与熊去氧胆酸治疗妊娠期肝内胆汁淤积症的疗效比较[J]. 肝脏, 2017, 22(8): 685-689.

LIAO E, SHAO Yong. Efficacy comparison between resveratrol and ursodeoxycholic acid in the treatment of intrahepatic cholestasis of pregnancy[J]. Chinese Hepatolgy, 2017, 22(8): 685-689.

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