肝细胞肝癌患者外周血中T细胞亚群和NK细胞的变化

肝脏 ›› 2016, Vol. 21 ›› Issue (5): 381-383.

肝细胞肝癌患者外周血中T细胞亚群和NK细胞的变化

李晓云, 于晓辉, 王维, 戴飞, 武承凤, 孙延荣

730050 甘肃兰州兰州军医兰州总医院医务部(李晓云,孙延荣),消化科(于晓辉,王维,武承凤),统计室(戴飞)

收稿日期:2014-12-10 出版日期:2016-05-31 发布日期:2020-07-09
通讯作者: 于晓辉,Email: yuxiaohui528@126.com

Received:2014-12-10 Online:2016-05-31 Published:2020-07-09

摘要/Abstract

摘要： 目的探讨肝细胞肝癌(HCC)外周血中T细胞亚群及NK细胞含量的变化,进一步了解HCC患者的免疫功能状况。方法收集181例HCC患者作为试验组,其中小HCC 39例、中等HCC 64例、大HCC 46例、巨块型HCC 32例;有肝硬化基础的HCC 150例,其中乙型肝炎肝硬化96例、丙型肝炎肝硬化49例、酒精性肝硬化3例、自身免疫性肝硬化2例。无肝硬化基础的HCC 31例,其中乙型肝炎病毒感染20例、丙型肝炎病毒感染8例、无原因的3例。对150例有肝硬化基础的患者按Child-Pugh分成A、B、C三级,其中A级37例、B级47例、C级66例。选择46名健康人群作为正常对照组。采用流式细胞仪检测两组患者血清中CD3⁺T细胞、CD4⁺T细胞、CD8⁺T细胞和NK细胞的含量。结果随着HCC体积的增大,外周血中CD3⁺T细胞、CD4⁺T细胞和NK细胞的含量明显降低,而CD8⁺T细胞含量升高,与正常对照组比较,其差异具有统计学意义(P<0.05);有肝硬化基础的HCC患者,随着Child-Pugh分级的增加, CD3⁺T细胞、CD4⁺T细胞和NK细胞的含量也明显降低,而CD8⁺T细胞含量升高,与正常对照组比较,差异具有统计学意义(P<0.05)。结论 HCC患者的T细胞和NK细胞的变化不但与肿瘤大小相关,还与HCC的肝硬化背景及分级相关。HCC患者机体免疫状况的变化,对判断HCC患者的免疫功能、病情及预后具有重要的临床意义。

关键词: 肝细胞肝癌, CD3⁺T细胞, CD4⁺T细胞, CD8⁺T细胞, NK细胞

李晓云, 于晓辉, 王维, 戴飞, 武承凤, 孙延荣. 肝细胞肝癌患者外周血中T细胞亚群和NK细胞的变化[J]. 肝脏, 2016, 21(5): 381-383.

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