基于Micro-CT成像对肝癌原位移植瘤血管三维结构的定量研究

肝脏 ›› 2020, Vol. 25 ›› Issue (12): 1290-1293.

基于Micro-CT成像对肝癌原位移植瘤血管三维结构的定量研究

刘巧遇, 李若坤

200072 同济大学附属第十人民医院放射科(刘巧遇);上海交通大学附属瑞金医院放射科(李若坤)

收稿日期:2020-06-03 出版日期:2020-12-31 发布日期:2021-02-26
基金资助:
国家自然科学基金资助项目(81501433)

Quantitative analysis on the three-dimensional structure of vascular of orthotopic transplanted liver cancer based on Micro-CT imaging

LIU Qiao-yu¹, LI Ruo-kun²

1. Departmen of Radiology, Shanghai Tenth People's Hospital, Tongji University School of Medcine,Shanghai 200072, China;
2. Department of Radiology, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China

Received:2020-06-03 Online:2020-12-31 Published:2021-02-26

摘要/Abstract

摘要： 目的基于Micro-CT成像定量肝癌裸鼠原位移植瘤血管的三维结构特征。方法采用雄性Balb/c裸小鼠20只,随机分为肝癌组(n=10)和正常对照组(n=10),肝癌组采用MHCC97H人肝癌细胞株构建原位移植瘤模型,正常对照组肝内注射同等量0.9%氯化钠溶液。造模21 d后进行目标血管铸型和Micro-CT扫描,重建血管图像,观察肝癌和正常肝脏血管的三维形态并定量分析血管密度特征、血管形态特征上的差异。结果正常肝脏血管呈树枝状逐级分支,走行自然;肝癌血管分布杂乱,形态不规则。定量分析结果显示,相比于正常肝脏,肝癌的血管占比[(5.57 ±1.16) %比(14.29 ± 2.89) %,P<0.01]、血管分支密度[(3.86±1.15)比(7.25±3.30),P=0.007]明显降低,平均半径缩小[(0.17±0.03) mm比(0.22±0.04) mm, P=0.004],平均DF[(1.20±0.01)比(1.16±0.00),P<0.01]、平均SOAM[(4.56±0.10) rad/mm比(4.33±0.20) rad/mm,P=0.004]增加,平均血管分支长度略缩短但差异无统计学意义(P>0.05)。结论基于Micro-CT的成像展示了肝癌原位移植瘤血管的三维结构,通过量化肝癌和健康肝脏的血管特征差异,可以进一步明确抑制肿瘤血液供应的潜在途径。

关键词: 肝癌, 原位移植瘤, Micro-CT, 定量分析

Abstract: Objective Using Micro-computed tomography (Micro-CT) imaging to quantify the three-dimensional (3D) structural characteristics of vascular in orthotopic transplanted tumor model of liver cancer in nude mice.Methods Twenty male Babl/c nude mice were randomly divided into the hepatocellular carcinoma (HCC) group (n=10) and the normal control group (n=10). Human liver cancer cell line MHCC97H was applied for constructing orthotopic transplantation tumor models in the HCC group. The same volume of saline was injected internally in the normal control group. After 21 days of modeling, the vascular casting and Micro-CT scan of the target vessel were used to reconstruct the blood vessel image. The characteristics of vascular density and morphological were analyzed quantitatively in liver cancer and normal liver tissues.Results The blood vessels of normal liver distributed regularly and ran orderly, while the vessels of HCC did not. Quantitative analysis showed that compared with normal liver, the vessel ratio [(5.57 ± 1.16) % vs (14.29 ± 2.89) %, P<0.001], the vascular branch density (3.86 ± 1.15 vs 7.25 ± 3.30, P=0.007) and the average radius (0.17 ± 0.03 mm vs 0.22 ± 0.04 mm, P=0.004) in HCC were significantly reduced; the average distance factor (1.20 ± 0.01 vs 1.16 ± 0.00, P<0.001) and the average sum of angles metric (4.56 ± 0.10 rad/mm vs 4.33 ± 0.20 rad/mm, P=0.004) were increased; the average vascular branch length was slightly shorter with no statistical significance (P>0.05).Conclusion This study showed the 3D structure of blood vessels in hepatic orthotopic transplanted tumor based on Micro-CT imaging, and indicated the potential ways for reducing tumor blood supply by quantitatively analyzing the characteristics of blood vessels in HCC and healthy liver tissues.

Key words: Liver cancer, Orthotopic transplantation tumor, Micro-computed tomography, Quantitative analysis

刘巧遇, 李若坤. 基于Micro-CT成像对肝癌原位移植瘤血管三维结构的定量研究[J]. 肝脏, 2020, 25(12): 1290-1293.

LIU Qiao-yu, LI Ruo-kun. Quantitative analysis on the three-dimensional structure of vascular of orthotopic transplanted liver cancer based on Micro-CT imaging[J]. Chinese Hepatolgy, 2020, 25(12): 1290-1293.

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