慢性丙型肝炎患者肝纤维化程度与TLR2、TLR4 mRNA的关系

肝脏 ›› 2021, Vol. 26 ›› Issue (7): 754-756.

慢性丙型肝炎患者肝纤维化程度与TLR2、TLR4 mRNA的关系

贾国昌, 刘春江, 刘东坡, 柯近, 郭敬超

462500 河南舞钢市人民医院检验科(贾国昌,刘春江,刘东坡,柯近),消化内科(郭敬超)

收稿日期:2021-03-20 出版日期:2021-07-31 发布日期:2021-09-02
通讯作者: 贾国昌,Email:hh232021@163.com
基金资助:
郑州市科技攻关项目(141PPTGG442)

Relationship between the degree of liver fibrosis and mRNA levels of TLR2 and TLR4 in patients with chronic hepatitis C

JIA Guo-chang¹, LIU Chun-jiang¹, LIU Dong-po¹, KE Jin¹, GUO Jing-chao²

1. Department of laboratory,
2. Department of Gastroenterology, People's Hospital of Wugang City, Henan 462500, China

Received:2021-03-20 Online:2021-07-31 Published:2021-09-02
Contact: JIA Guo-chang,Email:hh232021@163.com

摘要/Abstract

摘要： 目的研究慢性丙型肝炎患者肝纤维化程度与Toll样受体2(TLR2)、Toll样受体4(TLR4)转录体(mRNA)的关系。方法对2019年1月到2019年12月因慢性丙型肝炎病毒(HCV)感染来舞钢市人民医院接受治疗的185例患者进行选择纳入试验,收录进慢性丙型肝炎(CHC)组,另选取同期在本院进行体检的185例健康人进行对照研究,收录进对照组,比较两组TLR2 mRNA、TLR4 mRNA水平;按照肝纤维化程度将CHC患者分成F1组(n=35)、F2组(n=63)、F3组(n=58)、F4组(n=29),比较四组TLR2 mRNA、TLR4 mRNA水平;利用Spearman系数分析肝纤维化程度分级F1、F2、F3、F4与TLR2 mRNA、TLR4 mRNA水平的相关性。结果 CHC组TLR2 mRNA、TLR4 mRNA水平显著高于对照组,有显著差异(P<0.05)。比较CHC患者不同纤维化程度TLR2 mRNA、TLR4 mRNA水平F1组＜F2组＜F3组＜F4组,有显著差异(P<0.05)。经相关性分析,CHC患者肝纤维化程度分级F1、F2、F3、F4与TLR2 mRNA、TLR4 mRNA表达呈正相关(P<0.05)。结论 TLR2 mRNA、TLR4 mRNA水平会随CHC患者肝纤维化程度进展而不断升高。

关键词: 慢性病性肝炎, 肝纤维化, Toll样受体2, Toll样受体4

Abstract: Objective To investigate the relationship between the degree of liver fibrosis and messenger RNA (mRNA) levels of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4) in patients with chronic hepatitis C (CHC). Methods From January 2019 to December 2019, 185 patients with CHC treated in our hospital were enrolled as CHC group. Another 185 healthy individuals who underwent physical examination in our hospital during the same period were enrolled as control group. The mRNA levels of TLR2 and TLR4 were compared between the 2 groups. According to the degree of liver fibrosis, CHC patients were divided into F1 subgroup (n=35), F2 subgroup (n=63), F3 subgroup (n=58) and F4 subgroup (n=29). The levels of TLR2 mRNA and TLR4 mRNA were compared among the subgroups. Spearman's correlation coefficient was used to analyze the correlation between the degree of liver fibrosis and mRNA levels of TLR2 and TLR4. Results The levels of TLR2 mRNA and TLR4 mRNA in CHC group were significantly higher than those in control group (P<0.05). The levels of TLR2 mRNA and TLR4 mRNA were statistically different among subgroups with different degree of liver fibrosis (F1 subgrouP<F2 subgrouP<F3 subgrouP<F4 subgroup, P<0.05). Correlation analysis showed that the expression of TLR2 mRNA and TLR4 mRNA was positively correlated with the degree of liver fibrosis in CHC patients (P<0.05). Conclusion The levels of TLR2 mRNA and TLR4 mRNA increase with the progression of liver fibrosis in CHC patients.

Key words: Chronic viral hepatitis, Liver fibrosis, Toll-like receptor 2, Toll-like receptor 4

贾国昌, 刘春江, 刘东坡, 柯近, 郭敬超. 慢性丙型肝炎患者肝纤维化程度与TLR2、TLR4 mRNA的关系[J]. 肝脏, 2021, 26(7): 754-756.

JIA Guo-chang, LIU Chun-jiang, LIU Dong-po, KE Jin, GUO Jing-chao. Relationship between the degree of liver fibrosis and mRNA levels of TLR2 and TLR4 in patients with chronic hepatitis C[J]. Chinese Hepatolgy, 2021, 26(7): 754-756.

参考文献

[1] Ampuero J, Romero-Gomez M . Hepatitis c virus: current and evolving treatments for genotypes 2 and 3. Gastroenterol Clin North Am, 2015, 44:845-857.
[2] 赵治锋, 罗蒙. 叉头转录因子家族与肝纤维化的研究进展. 中华实验外科杂志, 2020, 37:1949-1955.
[3] Raby A C, Colmont C S, Kiftmorgan A, et al. Toll-like receptors 2 and 4 are potential therapeutic targets in peritoneal dialysis-associated fibrosis. J Am Soc Nephrol, 2017, 28:461-478.
[4] Bhattacharyya S, Midwood K S, Yin H, et al. Toll-like receptor-4 signaling drives persistent fibroblast activation and prevents fibrosis resolution in scleroderma.. Adv Wound Care, 2017, 6:356-369.
[5] 中华医学会肝病学分会,中华医学会感染病学分会.丙型肝炎防治指南(2015更新版).肝脏,2015, 20:933-949.
[6] Scheuer P J. Classification of chronic viral hepatitis: a need for reassessment. J Hepatol, 1991, 13:372-374.
[7] 李海, 杨庆利, 居昱,等. 广西苗族,侗族儿童乙肝疫苗初次免疫应答水平与TLR基因多态性的关联. 中国妇幼保健, 2019, 34:92-97.
[8] 闫静静, 王玮珺, 范慧倩,等. FibroTouch对自身免疫性肝病肝纤维化的诊断效能及其影响因素. 中华肝脏病杂志, 2020, 28:1048-1051.
[9] 文皑巍, 曹文富. Toll样受体信号通路在肝纤维化机制中的作用. 华西医学, 2015, 30:186-189.
[10] 池刚, 荣凯, 裴晋红,等. TLR2/4配体增强的非特异性肝脏炎症对BALB/c小鼠自身免疫性肝炎的影响. 中国病理生理杂志, 2019, 35:185-189.
[11] 杨超, 谭勤锐, 李晖. Toll样受体对肝纤维化的调控作用进展. 重庆医学, 2017, 46:2853-2856.
[12] 朱建光、沈露、周汉义,等. 慢性丙肝患者外周血单核细胞中TLR2,CTLA-4 mRNA的表达及意义. 山东医药, 2020, 60:5-9.
[13] 伍振辉, 孟娴, 胡佳伟,等. TLR4-MyD88-NF-kB信号通路与肝炎-肝纤维化-肝癌轴相关性研究进展. 国际药学研究杂志, 2017, 44:396-401.
[14] 许深深, 叶波平. Toll样受体与肝纤维化. 药物生物技术, 2014, 21:589-592.
[15] 马英杰. 肝硬化患者小肠细菌过度生长与外周血内毒素水平、TLR2、TLR4表达的关系. 医学研究杂志, 2018, 47:130-133.