急重症胰腺炎合并肝损伤患者IL-10、TGFβ及其mRNA水平与肝功能指标的相关性

摘要/Abstract

摘要： 目的分析急重症胰腺炎(SAP)合并肝损伤患者白细胞介素10(IL-10)、转化生长因子β(TGFβ)及其转录物(mRNA)水平与肝功能指标的相关性。方法收集2018年10月到2020年10月在东营市人民医院确诊并接受治疗的60例SAP患者纳入本次试验,根据其有无合并肝损伤分为合并组(合并肝损伤)及SAP(未合并肝损伤)组,每组30例,另纳入同期来我院进行体格检查的30名健康志愿者进行对照研究,纳入对照组,比较三组入院时IL-10、TGFβ、IL-10mRNA、TGFβmRNA水平及肝功能指标[ALT、AST、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、总胆红素(TBil)],通过Spearman系数分析其相关性。结果三组IL-10、IL-10 mRNA水平比较合并组＞SAP组＞对照组[分别为(56.74±4.24)、(47.58±6.74)比(39.85±4.36)ng/mL,(1.14±0.21)、(0.75±0.14)比(0.61±0.11)],合并组TGFβ、TGFβ mRNA水平显著高于SAP组、对照组[分别为(72.04±8.61)比(67.54±7.14)、(65.38±6.71)ng/L,(0.47±0.08)比(0.41±0.05)、(0.40±0.04)],差异均有统计学意义(P<0.05)。合并组AST、ALT、TBil、GGT、ALP水平显著高于SAP组、对照组[分别为(85.42±10.24)比(34.24±5.64)、(32.15±5.16)U/L,(79.46±10.41)比(35.15±4.26)、(33.41±4.18)U/L,(40.15±5.42)比(35.41±4.34)、(33.28±4.08)μmol/L,(61.04±7.18)比(56.34±5.48)、(54.23±4.35)U/L,(116.54±18.41)比(104.64±12.36)、(102.58±11.85)U/L],差异均有统计学意义(P<0.05)。SAP合并肝损伤患者IL-10、IL-10 mRNA、TGFβ、TGFβ mRNA水平与AST、ALT、GGT、ALP、TBil水平呈明显正相关(P<0.05)。结论 SAP合并肝损伤患者白介素10(IL-10)、TGFβ及其mRNA水平与肝功能指标存在明显相关性。

关键词: 急重症胰腺炎, 白细胞介素10, 炎症介质转化生长因子, 肝损伤

Abstract: Objective To analyze the correlation between the levels of interleukin-10 (IL-10), transforming growth factor - β (TGFβ) and its transcripts (mRNA) and liver function indexes in patients with severe acute pancreatitis (SAP) complicated with liver injury. Methods 60 SAP patients who were diagnosed and treated in our hospital from October 2018 to October 2020 were included in this trial. According to whether they had liver injury or not, they were divided into combined group (with liver injury) and sap (without liver injury) group, with 30 cases in each group, in addition, 30 healthy volunteers who came to our hospital for physical examination during the same period were included for a controlled study, and the control group was included.The levels of IL-10, TGFβ, IL-10 mRNA, TGFβ mRNA and liver function indexes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), total bilirubin (TBil)] of the three groups at admission were compared, and the correlation was analyzed by Spearman coefficient. Results The levels of IL-10 and IL-10 mRNA in the combined group were higher than those in the sap group and the control group[(56.74±4.24),(47.58±6.74) vs(39.85±4.36)ng/mL;(1.14±0.21),(0.75±0.14)vs(0.61±0.11)], The levels of TGF - β and TGF - β mRNA in the combined group were significantly higher than those in SAP group and control group[(72.04±8.61)vs(67.54±7.14),(65.38±6.71)ng/L; (0.47±0.08)vs(0.41±0.05),(0.40±0.04)], There was significant difference (P<0.05).The levels of AST, ALT, TBil, GGT and ALP in the combined group were significantly higher than those in SAP group and control group[(85.42±10.24)vs(34.24±5.64),(32.15±5.16)U/L;(79.46±10.41)vs(35.15±4.26),(33.41±4.18)U/L;(40.15±5.42)vs(35.41±4.34),(33.28±4.08)μmol/L,(61.04±7.18)vs(56.34±5.48)U/L,(54.23±4.35)U/L;(116.54±18.41)vs(104.64±12.36),(102.58±11.85)U/L],There was significant difference (P<0.05).The levels of IL-10, IL-10 mRNA, TGFβ and TGF - β mRNA were positively correlated with AST,ALT, GGT, ALP and TBil in SAP patients with liver injury (P<0.05). Conclusion The levels of interleukin-10 (IL-10), transforming growth factor β (TGFβ) and their transcripts (mRNA) were significantly correlated with liver function in SAP patients with liver injury.

Key words: Severe acute pancreatitis, Interleukin-10, Inflammatory mediators transforming growth factor, Liver injury

焦芳芳, 张伟, 武云珍. 急重症胰腺炎合并肝损伤患者IL-10、TGFβ及其mRNA水平与肝功能指标的相关性[J]. 肝脏, 2021, 26(9): 1027-1030.

JIAO Fang-fang, ZHANG Wei, WU Yun-zhen. Correlation analysis of IL-10, TGF - β and their mRNA levels with liver function indexes in patients with severe acute pancreatitis complicated with liver injury[J]. Chinese Hepatolgy, 2021, 26(9): 1027-1030.

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