肝细胞CK7染色阳性与门静脉肝窦血管病临床病理表型的相关性分析

肝脏 ›› 2026, Vol. 31 ›› Issue (1): 81-88.

肝细胞CK7染色阳性与门静脉肝窦血管病临床病理表型的相关性分析

单姗, 马琳, 曾馨, 王昱, 张冠华, 赵新颜

100050 北京首都医科大学附属北京友谊医院肝病中心,消化健康全国重点实验室,国家消化系统疾病临床医学研究中心

收稿日期:2025-09-09 出版日期:2026-01-31 发布日期:2026-03-30
通讯作者: 赵新颜,Email: zhao_xinyan@ccmu.edu.cn
基金资助:
北京整合医学学会临床科研资助计划项目ZHKY-2025-1869(C009)

Association between hepatocellular cytokeratin 7 expression and clinicopathological features of porto-sinusoidal vascular disease

SHAN Shan, MA Lin, ZENG Xin, WANG Yu, ZHANG Guan-hua, ZHAO Xin-yan

Liver Research Center, Beijing Friendship Hospital, Capital Medical University; State Key Laboratory of Digestive Health; National Clinical Research Center for Digestive Diseases, Beijing 100050, China

Received:2025-09-09 Online:2026-01-31 Published:2026-03-30
Contact: ZHAO Xin-yan, Email: zhao_xinyan@ccmu.edu.cn

摘要/Abstract

摘要： 目的探讨肝细胞细胞角蛋白7(CK7)阳性表达与门静脉肝窦血管病(PSVD)临床及病理表型的相关性,评估肝细胞CK7阳性在疾病进展及临床分层管理中的潜在价值。方法回顾性分析2003年1月至2022年7月在首都医科大学附属北京友谊医院确诊的PSVD患者临床及病理资料。根据肝细胞CK7免疫组化染色结果将患者分为阳性组与阴性组,比较两组的临床特征、生化指标、肝脏硬度测量值(LSM)及病理学表现,并随访不同CK7表达患者的预后。结果共纳入PSVD患者103例,其中CK7阳性21例(20.4%)。与CK7染色阴性患者相比,CK7阳性组碱性磷酸酶(中位数135.0 U/L vs. 76.4 U/L,P<0.001)与γ谷氨酰转移酶(51.0 U/L vs. 24.5 U/L,P=0.005)显著升高,白蛋白水平下降(34.0 g/L vs. 38.6 g/L,P=0.004),LSM升高(13.6 kPa vs. 8.7 kPa,P=0.004)。CK7阳性患者结节性再生性增生及窦周纤维化的发生率分别为33.3%和52.4%,均显著高于阴性组(P<0.001)。中位随访26个月,所有患者五年累计生存率为94.4%。CK7阳性与阴性患者在肝移植/死亡及门静脉高压并发症(如曲张静脉出血、腹水)方面的发生率差异无统计学意义。结论 PSVD患者中,肝细胞CK7阳性表达与胆汁淤积性生化异常、肝脏硬度升高、结节性再生性增生及窦周纤维化密切相关,提示CK7阳性反映肝组织应激性和再生性重构活跃的病理亚型。CK7阳性有望作为评估PSVD组织学异质性及疾病进展的潜在标志物。

关键词: 细胞角蛋白7, 门脉肝窦血管病, 胆汁淤积, 窦周纤维化

Abstract: Objective To investigate the correlation between hepatic cytokeratin 7 (CK7) expression and the clinicopathological features of porto-sinusoidal vascular disease (PSVD), and to evaluate the potential role of CK7 positivity in disease progression and clinical stratification. Methods A retrospective study was conducted on patients diagnosed with PSVD at Beijing Friendship Hospital, Capital Medical University, between January 2003 and July 2022. Patients were categorized into CK7-positive and CK7-negative groups based on immunohistochemical staining of hepatocytes. Clinical characteristics, biochemical parameters, liver stiffness measurement (LSM), and histological features were compared between groups. Prognostic outcomes were analyzed during follow-up. Results A total of 103 PSVD patients were included, with 21 (20.4%) showing CK7 positivity in hepatocytes. Compared with CK7-negative patients, the CK7-positive group exhibited higher levels of alkaline phosphatase (135.0 vs 76.4 U/L, P<0.001) and γ-glutamyl transferase (51.0 vs 24.5 U/L, P=0.005), lower albumin levels (34.0 vs 38.6 g/L, P=0.004), and higher LSM values (13.6 vs 8.7 kPa, P=0.004). Histologically, nodular regenerative hyperplasia and perisinusoidal fibrosis were more frequent in the CK7-positive group (33.3% and 52.4%, respectively; both P<0.001). During a median follow-up of 26 months, the five-year cumulative survival rate was 94.4%, with no significant difference in liver transplantation/death or portal hypertension-related complications between groups. Conclusion Hepatocellular CK7 positivity in PSVD is associated with cholestatic biochemical abnormalities, increased liver stiffness, nodular regenerative hyperplasia, and perisinusoidal fibrosis. CK7-positive expression may indicate a more active hepatocellular regenerative state and serve as a potential histological marker for assessing tissue heterogeneity and disease progression in PSVD.

Key words: Cytokeratin 7, Portosinusoidal vascular disease, Cholestasis, Perisinusoidal fibrosis

单姗, 马琳, 曾馨, 王昱, 张冠华, 赵新颜. 肝细胞CK7染色阳性与门静脉肝窦血管病临床病理表型的相关性分析[J]. 肝脏, 2026, 31(1): 81-88.

SHAN Shan, MA Lin, ZENG Xin, WANG Yu, ZHANG Guan-hua, ZHAO Xin-yan. Association between hepatocellular cytokeratin 7 expression and clinicopathological features of porto-sinusoidal vascular disease[J]. Chinese Hepatolgy, 2026, 31(1): 81-88.

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