HBV相关慢加急性肝衰竭前期外周血中Toll样受体3、4的表达

肝脏 ›› 2019, Vol. 24 ›› Issue (5): 499-502.

HBV相关慢加急性肝衰竭前期外周血中Toll样受体3、4的表达

苏金良, 陈慧, 朱莉, 陆敏嘉, 李明

215007 江苏苏州市第五人民医院、苏州大学附属传染病医院感染科

收稿日期:2018-12-24 发布日期:2020-04-10
通讯作者: 李明, Email:llttyy97@163.com
基金资助:
苏州市民生科技-医疗卫生应用基础研究计划项目(SYS201657)

Expression of toll-like receptor 3 and 4 in peripheral blood mononuclear cells from patients with hepatitis B virus -related acute-on-chronic pre-liver failure

SU Jin-liang, CHEN Hui, ZHU Li, LU Min-Jia, LI Ming

The Fifth People’s Hospital of Suzhou, the Affiliated Infectious Diseases Hospital of Soochow University, Suzhou 215007,China

Received:2018-12-24 Published:2020-04-10
Contact: LI Ming, Email: llttyy97@163.com

摘要/Abstract

摘要： 目的探讨HBV相关慢加急性肝衰竭前期(pre-ACLF)外周血单个核细胞中Toll样受体3(TLR3)和Toll样受体4(TLR4)表达水平的变化及其意义。方法采用流式细胞仪检测HBV相关的pre-ACLF患者30例、慢性乙型肝炎患者30例以及健康对照者15名外周血单个核细胞TLR3、TLR4的表达水平,绘制ROC曲线,评价TLR3和TLR4对pre-ACLF的诊断价值。结果 pre-ACLF组患者外周血中TLR3(20.81±5.15)明显升高,分别与慢性乙型肝炎组、健康对照组比较,均差异有统计学意义(均P<0.05)。同时,pre-ACLF组患者外周血中TLR4(13.73±3.56)也明显上升,分别与慢性乙型肝炎组、健康对照组比较,也均差异有统计学意义(均P<0.05)。根据TLR3和TLR4检测结果分别绘制ROC曲线, 外周血TLR3诊断pre-ACLF的ROC曲线下的面积(AUC)为 0.830(95％CI为0.728～0.932 );外周血TLR4诊断pre-ACLF的ROC曲线下面积为0.977(95％CI为0.948～1.000)。结论通过对pre-ACLF患者TLR3和TLR4水平的检测,有助于对pre-ACLF的诊断。

关键词: Toll样受体3, Toll样受体4, 肝衰竭, 前期, 乙型肝炎病毒, 诊断

Abstract: Objective To investigate the changes and significance of the expression level of toll-like receptor 3 (TLR3) and toll-like receptor 4 (TLR4) in peripheral blood mononuclear cells (PBMC) from patients with hepatitis B virus (HBV)-related acute-on-chronic pre-liver failure (pre-ACLF).Methods The expression of TLR3 and TLR4 in PBMC from 30 patients with pre-ACLF, 30 patients with chronic hepatitis B (CHB) and 15 healthy controls was detected by flow cytometry. Meanwhile, the diagnostic value of TLR3 and TLR4 in pre-ACLF was evaluated by the receiver operating characteristic (ROC) curves.Results The levels of TLR3 and TLR4 from PBMC in pre-ACLF group were significantly higher than those in CHB and control groups (P<0.05). The area under ROC curves of TLR3 and TRL4 were 0.830 (95% confidence interval: 0.728 ~ 0.932) and 0.977 (95% confidence interval: 0.948 ~ 1.000), respectively.Conclusion Detection the levels of TLR3 and TLR4 may contribute to the diagnosis of HBV-related pre-ACLF.

Key words: Toll-like receptor 3, Toll-like receptor 4, Liver failure, Hepatitis B virus, Prophase, Diagnosis

苏金良, 陈慧, 朱莉, 陆敏嘉, 李明. HBV相关慢加急性肝衰竭前期外周血中Toll样受体3、4的表达[J]. 肝脏, 2019, 24(5): 499-502.

SU Jin-liang, CHEN Hui, ZHU Li, LU Min-Jia, LI Ming. Expression of toll-like receptor 3 and 4 in peripheral blood mononuclear cells from patients with hepatitis B virus -related acute-on-chronic pre-liver failure[J]. Chinese Hepatolgy, 2019, 24(5): 499-502.

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