转录因子Slug与KLF5相互作用及其对肝癌细胞迁移能力的影响

肝脏 ›› 2018, Vol. 23 ›› Issue (5): 395-399.

转录因子Slug与KLF5相互作用及其对肝癌细胞迁移能力的影响

严云青, 王志良

201999 上海交通大学医学院附属第九人民医院输血科

收稿日期:2018-03-10 出版日期:2018-05-31 发布日期:2020-04-28
通讯作者: 王志良,Email:13816735502@139.com

Interaction between transcription factor Slug and KLF5 and its effect on the migration of hepatoma cells

YAN Yun-qing, WANG Zhi-liang

Department of Blood Transfusion, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 201999, China

Received:2018-03-10 Online:2018-05-31 Published:2020-04-28
Contact: WANG Zhi-liang, Email: 13816735502@139.com

摘要/Abstract

摘要： 目的探讨转录因子Slug与KLF5之间的相互作用及Slug是否作为KLF5的下游信号因子影响肝癌细胞的迁移能力。方法通过免疫沉淀实验和免疫荧光实验检测Slug与KLF5之间相互作用及共定位,并在肝癌细胞中转入Slug过表达质粒,以及使用shRNA抑制其表达,观察肝癌细胞迁移能力的改变。结果在肝癌细胞中发现转录因子Slug的表达随KLF5的改变而改变,并且KLF5与Slug之间在蛋白水平存在相互作用及共定位。然而,在肝癌细胞中过表达或抑制Slug的表达并没有影响肝癌细胞的迁移能力。结论 KLF5与另一转录因子Slug存在相互作用及细胞中共定位,但是转录因子Slug并不是作为KLF5的下游信号因子影响肝癌细胞的迁移能力。

关键词: 转录因子, KLF5, Slug, 肝细胞癌, 迁移

Abstract: Objective To investigate the interaction between transcription factor Slug and Krüpple-like factor 5 (KLF5), and whether Slug acts as a downstream signaling factor of KLF5 and affect the migration ability of hepatocellular carcinoma (HCC) cells. Methods The interaction and co-localization between Slug and KLF5 were detected by immunoprecipitation and immunofluorescence assay. Slug overexpression plasmid was transfected into HCC cells and shRNA was used to inhibit the expression of Slug in HCC cells. Migration ability of HCC cells was observed. Results In HCC cells, the expression of transcription factor Slug was found altering with the change of KLF5, and their interaction and co-localization were at protein level. However, upregulation or downregulation of Slug expression in HCC cells did not affect the migration ability of HCC cells. Conclusion The transcription factor KLF5, which is known to inhibit the migration of HCC cells, is found to interact with another transcription factor Slug and has cell co-localization with Slug. However, transcription factor Slug is not a downstream signaling factor of KLF5 and cannot affect the migration ability of HCC cells.

Key words: Transcription factor, KLF5, Slug, Hepatocellular carcinoma, Migration

严云青, 王志良. 转录因子Slug与KLF5相互作用及其对肝癌细胞迁移能力的影响[J]. 肝脏, 2018, 23(5): 395-399.

YAN Yun-qing, WANG Zhi-liang. Interaction between transcription factor Slug and KLF5 and its effect on the migration of hepatoma cells[J]. Chinese Hepatolgy, 2018, 23(5): 395-399.

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