慢性乙型肝炎患者天然免疫相关基因表达差异研究

摘要/Abstract

摘要： 目的观察慢性乙型肝炎患者(CHB)天然免疫相关基因表达差异。方法选取2017年6月至2018年6月本院收治的CHB患者174例和健康者40例(H组)。根据CHB诊断标准分组:慢性HBV患者48例(A组),HBeAg阳性CHB患者44例(B组),HBeAg阴性CHB患者40例(C组),非活动性HBsAg阴性CHB患者42例(D组)。分离外周血单核细胞,提取细胞RNA,实时定量RT-PCR检测31个天然免疫相关基因表达变化。结果四组CHB患者和H组的IFN及其受体、IRF、TLR、干扰素诱导基因mRNA表达水平差异均有统计学意义(P<0.05)。A、B、C、D组IFNλ、IL10RB、IFN γR2、IRF7、IRF9、TLR4、MYD88均显著高于H组(P<0.05);A、B、C、D组IFNRλ1均低于H组(P<0.05)。不同CHB组别IFNβ、IFNγ、IFNλ、IFN αR2、IFNRλ1、IRF4、IRF5、IRF8、TLR2、TLR8、TLR9、JAK1、RIG1、TRIF、TRAF6、IRAK1、MAVS差异具有统计学意义(P<0.05)。31个检测基因中,A组20个基因相对表达高于H组2.5倍以上,B组11个,C组19个,D组8个,差异有统计学意义(χ²=13.605,P=0.003)。A组1个基因相对表达低于H组0.5倍以上,B组9个,C组2个,D组13个,差异有统计学意义(χ²=19.790,P=0.001)。结论 CHB患者天然免疫应答基因在CHB疾病进展期间差异表达,这种差异可能有助于开发新的非侵入性方法来确定慢性HBV患者的疾病进展阶段。

关键词: 慢性乙型肝炎, 干扰素, 干扰素调节因子, Toll样受体, 干扰素诱导基因

曹蓉, 杜阿妮. 慢性乙型肝炎患者天然免疫相关基因表达差异研究[J]. 肝脏, 2019, 24(10): 1144-1147.

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