MicroRNA4293 基因多态性与女性肝癌易感性相关

肝脏 ›› 2016, Vol. 21 ›› Issue (5): 347-350.

MicroRNA4293 基因多态性与女性肝癌易感性相关

蔡敏, 郑万威, 张骏, 程烽涛, 李力

200090 上海同济大学附属杨浦医院消化科(蔡敏,程烽涛,李力);复旦大学附属华山医院消化科(郑万威,张骏)

收稿日期:2016-03-31 出版日期:2016-05-31 发布日期:2020-07-09
通讯作者: 李力,Email: lli10437@sina.com

Genetic polymorphisms of microRNA-4293 and hepatocellular carcinoma susceptibility in female

CAI Min, ZHENG Wan-wei, ZHANG Jun, CHENG Feng-tao, LI Li

Tongji University School of Medicine, Yangpu Hospital, Department of Digestive Diseases, Shanghai 200090, China

Received:2016-03-31 Online:2016-05-31 Published:2020-07-09
Contact: LI Li, Email: lli10437@sina.com

摘要/Abstract

摘要： 目的研究microRNA-4293的SNP(rs12220909)与原发性肝癌易感性的关系。方法收集人体血液样本1788份,样本取自健康对照者884名,原发性肝癌患者904例。抽提血液样本中基因组DNA,应用MassARRAY法对microRNA-4293的SNP(rs12220909)位点进行基因分型;应用二元逻辑回归法分析microRNA-4293的SNP(rs12220909)位点与肝癌易感性之间的关系。结果女性肝癌患者microRNA-4293的SNP(rs12220909)中CC基因型较健康对照组显著增加(P=0.04),在HBV相关肝癌中显著性进一步增加(P=0.02),提示CC基因可能增加女性肝癌尤其是HBV相关肝癌的易感性。而在男性患者中,microRNA-4293的SNP(rs12220909)与肝癌易感性并不相关(P=0.33)。结论 microRNA-4293的SNP(rs12220909)与女性肝癌易感性相关。

关键词: 原发性肝癌, 易感性, MicroRNA-4923, SNP(rs12220909)

Abstract: Objective To investigate the association between single nucleotide polymorphism (SNP) mapping to microRNA-4293 (rs12220909) and hepatocellular carcinoma (HCC) susceptibility.Methods Blood samples were collected from 1788 cases, including 884 normal control cases and 904 HCC cases. Genomic deoxyribonucleic acid was extracted from blood samples, and SNPs mapping to microRNA-4293 (rs12220909) were detected by MassARRAY. Then association between genotype of rs12220909 and HCC susceptibility was analyzed by binary logistic regression.Results In female HCC patients, CC genotype of rs12220909, especially in hepatitis B virus (HBV)-related HCC patients (P=0.02), was significantly higher than that in healthy control group (P=0.04). It indicated that CC genotype might be a risk factor for female HCC, especially for HBV-related HCC. However, this association did not exist in male patients (P=0.33).Conclusion Genotype of microRNA-4293 (rs12220909) is relevant to HCC in female patients.

Key words: Hepatocellular carcinoma; Susceptibility; MicroRNA-4923; SNP(rs12220909)

蔡敏, 郑万威, 张骏, 程烽涛, 李力. MicroRNA4293 基因多态性与女性肝癌易感性相关[J]. 肝脏, 2016, 21(5): 347-350.

CAI Min, ZHENG Wan-wei, ZHANG Jun, CHENG Feng-tao, LI Li. Genetic polymorphisms of microRNA-4293 and hepatocellular carcinoma susceptibility in female[J]. Chinese Hepatolgy, 2016, 21(5): 347-350.

参考文献

[1] El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 2007, 132:2557-2576.
[2] Hou W, Bonkovsky HL. Non-coding RNAs in hepatitis C-induced hepatocellular carcinoma: dysregulation and implications for early detection, diagnosis and therapy. World J Gastroenterol 2013, 19:7836-7845.
[3] de Lope CR, Tremosini S, Forner A, et al. Management of HCC. J Hepatol, 2012, 56 (Suppl 1):S75-S87.
[4] Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell, 2009, 136:215-233.
[5] Wang XW, Heegaard NH, Orum H: MicroRNAs in liver disease. Gastroenterology, 2012, 142:1431-1443.
[6] Chung CC, Chanock SJ. Current status of genome-wide association studies in cancer. Hum Genet, 2011, 130:59-78.
[7] Pastinen T, Ge B, Hudson TJ. Influence of human genome polymorphism on gene expression. Hum Mol Genet, 2006, 15:R9-R16.
[8] Selbach M, Schwanhausser B, Thierfelder N, et al. Widespread changes in protein synthesis induced by microRNAs. Nature, 2008, 455:58-63.
[9] Zhang P, Wang J, Lu T, et al. MiR-449b rs10061133 and miR-4293 rs12220909 polymorphisms are associated with decreased esophageal squamous cell carcinoma in a Chinese population. Tumour Biol, 2015, 36:8789-8795.
[10] Wang X, Zhang A, Sun H. Power of metabolomics in diagnosis and biomarker discovery of hepatocellular carcinoma. Hepatology, 2013, 57:2072-2077.
[11] Wen J, Friedman JR. miR-122 regulates hepatic lipid metabolism and tumor suppression. J Clin Invest, 2012, 122:2773-2776.
[12] Diaz G, Melis M, Tice A, et al. Identification of microRNAs specifically expressed in hepatitis C virus-associated hepatocellular carcinoma. Int J Cancer, 2013, 133:816-824.
[13] Chen JS, Wang Q, Fu XH, et al. Involvement of PI3K/PTEN/AKT/mTOR pathway in invasion and metastasis in hepatocellular carcinoma: Association with MMP-9. Hepatol Res, 2009, 39:177-186.
[14] Hsu HC, Tseng HJ, Lai PL, et al. Expression of p53 gene in 184 unifocal hepatocellular carcinomas: association with tumor growth and invasiveness. Cancer Res, 1993, 53:4691-4694.
[15] Li XD, Li ZG, Song XX, et al. A variant in microRNA-196a2 is associated with susceptibility to hepatocellular carcinoma in Chinese patients with cirrhosis. Pathology, 2010, 42:669-673.
[16] Qi P, Dou TH, Geng L, et al. Association of a variant in MIR 196A2 with susceptibility to hepatocellular carcinoma in male Chinese patients with chronic hepatitis B virus infection. Hum Immunol, 2010, 71:621-626.
[17] Zhang J, Wang R, Ma YY, et al. Association between single nucleotide polymorphisms in miRNA196a-2 and miRNA146a and susceptibility to hepatocellular carcinoma in a Chinese population. Asian Pac J Cancer Prev, 2013, 14:6427-6431.
[18] Zhou J, Yu L, Gao X, et al. Plasma microRNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma. J Clin Oncol, 2011, 29:4781-4788.
[19] Zhang QH, Sun HM, Zheng RZ, et al. Meta-analysis of microRNA-183 family expression in human cancer studies comparing cancer tissues with noncancerous tissues. Gene, 2013, 527:26-32.