IL-9在免疫性肝损伤患者的表达及临床意义

肝脏 ›› 2017, Vol. 22 ›› Issue (10): 904-906.

IL-9在免疫性肝损伤患者的表达及临床意义

王兰,张丽,杨瑞宁

210002 江苏南京南京中医药大学附属南京八一医院检验科

收稿日期:2016-12-17 出版日期:2017-10-15 发布日期:2020-06-16
通讯作者: 杨瑞宁,E-mail:jsnjyrn@163.com

Received:2016-12-17 Online:2017-10-15 Published:2020-06-16

摘要/Abstract

摘要： 目的比较免疫性肝损伤患者与健康人外周血中IL-9的含量差异,探讨IL-9在免疫性肝损伤中的作用及可能机制。方法选取乙型肝炎患者45例,自身免疫性肝病患者20例以及健康对照组20名,采用酶联免疫分析(ELISA)及实时荧光定量逆转录PCR(RT-PCR)的方法检测各组外周血中的IL-9水平。结果与健康对照组相比,乙型肝炎组及自身免疫性肝病组的IL-9含量及IL-9 mRNA水平明显升高(P<0.05);乙型肝炎组的IL-9含量及IL-9 mRNA水平与自身免疫性肝病组相比略有升高,但差异无统计学意义。结论 IL-9可能在免疫性肝损伤的发病过程中发挥了重要作用。

王兰,张丽,杨瑞宁. IL-9在免疫性肝损伤患者的表达及临床意义[J]. 肝脏, 2017, 22(10): 904-906.

参考文献

[1] Veldhoen M,Uyttenhove C,van Snick J,et al.Transforming growth factor-beta‘reprograms’the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset.Nat Immunol,2008,9:1341-1346.
[2] Dardalhon V,Awasthi A,Kwon H,et al.IL-4 inhibits TGF-beta-induced Foxp3+ T cells and,together with TGF-beta,generates IL-9+IL-10+Foxp3(-) effector T cells.Nat lmmunol,2008,9:1347-1355.
[3] Louahed J,Kermouni A,Van Snick J,et al.IL-9 induces expression of granzymes and high-affinity lgE receptor in murine T helper clones.J Immunol,1995,154:5061-5070.
[4] Gounni AS,Gregory B,Nutku E,et al. IL-9 enhances interleukin-5 receptor expression,differentiation and survival of humman eosinophils.Blood,2000,96:2163-2171.
[5] Abdelilah S, Latifa K, Esra N, et al.Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: role in IL-8 release.J Immunol,2001,166:2768-2774.
[6] Little FF,Cruikshank WW,Center DM.IL-9 stimulates release of chemotactic factors from human bronchial epithelial cells.Am J Respir Cell Mol Biol,2001,25:347-352.
[7] Renauld JC,Druez C,Kermouni A,et al.Expression cloning of the murine and human interleukin 9 receptor cDNAs.Proc Natl Acad Sci U S A,1992,89:5690-5694.
[8] Demoulin JB,Van Roost E,Stevens M,et al.Distinct roles for STAT1,STAT3,and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9？J Biol Chem,1999,274:25855-25861.
[9] Louahed J,Zhou Y,Maloy WL,et al.Interleukin 9 promotes influx and local maturation of eosinophils.Blood,2001,97:1035-1042.
[10] Leech MD,Grencis RK. Induction of enhanced immunity to intestinal nematodes using IL-9-producting dendritic cells.immunol,2006,176:2505-2511.
[11] Nowak EC,Weaver CT,Turner H,et al.IL-9 as a mediator of Th17-driven inflammatory disease.J Exp Med,2009,206:1653-1660.
[12] Jager A,Dardalhon V,Sobel RA,et al.Th1,Th17,and Th9 effector cells induce experimental autoimmune encephalomyelitis with different pathological phenotypes. J Immunol, 2009, 183:7169-7177.
[13] de Vries VC,Wasiuk A,Bennett KA,et al.Mast cell degranulation breaks peripheral tolerance.Am J Transplant,2009,9:2270-2280.
[14] 刘肄辉,张永华,刘莉,等.辅助性T淋巴细胞9/调节性T淋巴细胞平衡在乙型肝炎病毒感染炎性反应中的变化及其意义.中华传染病杂志,2013,31:646-649.
[15] Jimenez-Sousa MA,Almansa R,de la Fuente C,et al.Increased Th1,Th17 and pro-fibrotic responses in hepatitis C-infected patients are down-regulated after 12 weeks of treatment with pegylated interferon plus ribavirin.Eur Cytokine Netw,2010,21:84-91.
[16] Nan XP,Zhang Y,Yu HT,et al.Inhibition of viral replication downregulates CD4(+)CD25(high)regulatory T cells and programmed death-ligand 1 in chronic hepatitis B.Viral Immunol,2012,25:21-28.