DKK3在非酒精性脂肪性肝病的作用研究

肝脏 ›› 2017, Vol. 22 ›› Issue (5): 414-417.

DKK3在非酒精性脂肪性肝病的作用研究

谢兰丰,沈婷婷,郑册,王丕晓,魏巧芳,杨长青,张琴

200065 上海同济大学医学院附属同济医院消化科(谢兰丰,杨长青);
上海交通大学医学院附属同仁医院感染科(谢兰丰,沈婷婷,郑册,张琴);
武汉大学心血管病研究所(王丕晓,魏巧芳)

出版日期:2017-05-30 发布日期:2017-05-30
通讯作者: 杨长青,Email:2008xielanfeng@163.com;张琴,Email:Zhangq1030@163.com
基金资助:
上海市公共卫生三年行动计划重点学科建设项目传染病与卫生微生物学(15GWZK0102)

Role of DKK3 in non-alcoholic fatty liver disease of mice model

XIE Lan-feng^1,2, SHEN Ting-ting², ZHENG Ce², WANG Pi-xiao³, WEI Qiao-fang³, YANG Chang-qing¹, ZHANG Qin²

1. Division of Gastroenterology and Hepatology,Tongji Hospital,Tongji University School of Medicine,Shanghai;
2. Department of Infectious Diseases, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;
3. Cardiovascular Research Institute, Wuhan University, Wuhan, China

Online:2017-05-30 Published:2017-05-30
Contact: ZHANG Qin, Email: Zhangq1030@163.com

摘要/Abstract

摘要： 目的探讨Dickkopf-3(DKK3)在小鼠非酒精性脂肪性肝病(NAFLD)形成中的意义。方法采用高脂饮食喂养C57BL/6雄性小鼠来构建NAFLD模型,通过检测肝功能、糖代谢变化、肝脏组织中脂质含量及肝脏病理学变化评估NAFLD模型是否成功;并用Western印迹方法检测肝脏组织中DKK3蛋白变化情况。结果高脂饮食组小鼠NAFLD相关指标均较对照组升高,差异有统计学意义(P<0.05)。高脂饮食组小鼠肝脏中DKK3蛋白表达量较对照组明显下降,定量分析比较差异有统计学意义(P<0.05)。结论 DKK3在NAFLD小鼠肝组织中表达明显下降,提示DKK3涉及NAFLD的发生发展过程,并可能发挥一定的作用。

关键词: 非酒精性脂肪性肝病, 小鼠模型, DKK3

Abstract: Objective To investigate the role of Dickkopf-3 (DKK3) in mice with non-alcoholic fatty liver disease (NAFLD).Methods Male C57BL/6 mice were applied to establish NAFLD model with high fat diet (HFD), which was validated using detections of hepatic function, glucose metabolism, lipid content and liver pathology. DKK3 expression in liver were measured using western blot.Results In HFD group, NAFLD-related parameters were significantly elevated, while hepatic DKK product was significantly decreased compared with that in control group.Conclusion Decreased expression of hepatic DKK3 in NAFLD mice model suggests that DKK3 might play a role in the development of NAFLD.

Key words: NAFLD, Mice model, DKK3

谢兰丰,沈婷婷,郑册,王丕晓,魏巧芳,杨长青,张琴. DKK3在非酒精性脂肪性肝病的作用研究[J]. 肝脏, 2017, 22(5): 414-417.

XIE Lan-feng, SHEN Ting-ting, ZHENG Ce, WANG Pi-xiao, WEI Qiao-fang, YANG Chang-qing, ZHANG Qin. Role of DKK3 in non-alcoholic fatty liver disease of mice model[J]. Chinese Hepatolgy, 2017, 22(5): 414-417.

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