Tfh细胞及其转录因子Ascl2在慢性乙型肝炎感染中的作用

肝脏 ›› 2018, Vol. 23 ›› Issue (12): 1069-1072.

Tfh细胞及其转录因子Ascl2在慢性乙型肝炎感染中的作用

王亚宁, 范超, 申焕君, 郝春秋, 贾战生

710038 西安第四军医大学唐都医院感染科

收稿日期:2018-06-30 发布日期:2020-04-29
通讯作者: 贾战生,Email:jiazsh@fmmu.edu.cn
基金资助:
国家自然科学基金资助项目(81471541)

The role of Tfh cells and its transcription factor achaete-scute homologue 2 in chronic infection of hepatitis B virus

WANG Ya-ning, FAN Chao, SHEN Huan-jun, HAO Chun-qiu, JIA Zhan-sheng

Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China

Received:2018-06-30 Published:2020-04-29
Contact: JIA Zhan-sheng,Email:jiazsh@fmmu.edu.cn

摘要/Abstract

摘要： 目的探讨滤泡辅助性T淋巴细胞(follicular helper T lymphocyte,Tfh)细胞及其转录因子无刚毛鳞甲复合体样蛋白2(achaete-scute homologue 2,Ascl2)在慢性HBV感染中的表达和意义。方法选择CHB患者40例,健康对照者30名。采用流式细胞术检测CD4⁺CXCR5⁺ Tfh细胞及CD4⁺CXCR5⁺PD-1⁺ Tfh细胞频数;实时定量 PCR 法检测Tfh细胞中 Ascl2表达水平;ELISA检测血清中各细胞因子水平。结果与健康对照者相比:CHB患者的CD4⁺CXCR5⁺ Tfh及CD4⁺CXCR5⁺PD-1⁺ Tfh细胞频数升高,差异有统计学意义(t=4.786,P<0.05);CHB患者中Ascl2 mRNA表达升高,差异有统计学意义 (t=4.655,P<0.05);CHB患者的IL-21、IL-17均升高,差异均有统计学意义(t=4.896和 t=3.764,P<0.05)。结论 Ascl2可能与HBV感染者Tfh细胞的分化发育有关。

关键词: 滤泡辅助性T淋巴细胞, 慢性乙型肝炎, 无刚毛鳞甲复合体样蛋白2

Abstract: Objective To investigate the significance of achaete-scute homologue 2 (Ascl2) expression in T follicular helper (Tfh) cells during chronic hepatitis B virus infection. Methods Frequencies of CD4⁺CXCR5⁺ Tfh cells and CD4⁺CXCR5⁺PD-1⁺ Tfh cells in peripheral blood mononuclear cells (PBMC) were analyzed by flow cytometry, respectively. The expression of Ascl2 in Tfh cells was determined by quantitative real-time polymerase chain reaction. The levels of serum interleukin-21 (IL-21) and interleukin-17 (IL-17) were measured using enzyme-linked immunosorbent assay. Results The frequencies of CD4⁺CXCR5⁺ and CD4⁺CXCR5⁺PD-1⁺ Tfh cells were significantly higher in PBMC from chronic hepatitis B (CHB) patients than that from healthy controls (t=4.786, P<0.05). The expression of Ascl2 was significantly higher in Tfh cells of CHB patients (t=4.655, P<0.05). Furthermore, the levels of serum IL-21 and IL-17 were significantly higher in CHB patients (t=4.896 and t=3.764, P<0.05). Conclusion It was implied that transcription factor Ascl2 plays an important role in the differentiation and development of Tfh cells in CHB patients.

Key words: Chronic hepatitis B; T follicular helper; Achaete-scute homologue 2

王亚宁, 范超, 申焕君, 郝春秋, 贾战生. Tfh细胞及其转录因子Ascl2在慢性乙型肝炎感染中的作用[J]. 肝脏, 2018, 23(12): 1069-1072.

WANG Ya-ning, FAN Chao, SHEN Huan-jun, HAO Chun-qiu, JIA Zhan-sheng. The role of Tfh cells and its transcription factor achaete-scute homologue 2 in chronic infection of hepatitis B virus[J]. Chinese Hepatolgy, 2018, 23(12): 1069-1072.

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