原发性肝癌患者外周血单个核细胞线粒体DNA含量检测及其临床意义

肝脏 ›› 2021, Vol. 26 ›› Issue (5): 514-517.

原发性肝癌患者外周血单个核细胞线粒体DNA含量检测及其临床意义

廖新爱, 冯惠娟, 卓传尚, 叶治, 柳丽娟

350025 福州福建医科大学孟超肝胆医院检验科

收稿日期:2020-09-02 出版日期:2021-05-31 发布日期:2021-06-17
通讯作者: 柳丽娟,Email:ljliu@126.com
基金资助:
福建省卫生计生科研人才培养项目(2018-CXB-18);国家十三五“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题(2018ZX10302205-003)

Detection and clinical significance of mitochondrial DNA content in peripheral blood mononuclear cells of patients with primary liver cancer

LIAO Xin-ai, FENG Hui-juan, ZHUO Chuan-shang, YE Zhi, LIU Li-juan

Department of Clinical Laboratory, the Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China

Received:2020-09-02 Online:2021-05-31 Published:2021-06-17
Contact: LIU Li-juan, Email:ljliu@126.com

摘要/Abstract

摘要： 目的探讨原发性肝癌(PLC)患者外周血单个核细胞(PBMC)线粒体DNA(mtDNA)的含量对肝癌诊断的临床意义。方法收集250例PLC患者和48例健康人群全血样本、临床信息及实验室指标等,实时荧光定量PCR检测线粒体中NADH脱氢酶亚基1(ND1)基因的含量,并以核基因组的β-globin作为内参基因。比较两组PBMC mtDNA含量的差异,同时比较分析mtDNA、甲胎蛋白(AFP)及二者联合诊断肝癌的效能。结果 PLC患者PBMC mtDNA含量(90.51)明显低于健康对照组(456.67)。诊断效能分析显示,mtDNA的曲线下面积(0.884)及灵敏度(75.20%)略高于AFP(0.863,71.70%);mtDNA与AFP联合诊断的曲线下面积为0.957,显著高于mtDNA(0.884)或AFP(0.866)单独检测,联合检测的灵敏度提高至84.91%。结论肝癌患者外周血PBMC mtDNA含量可作为肝癌诊断的辅助指标。

关键词: 原发性肝癌, 外周血单个核细胞, 线粒体DNA

Abstract: Objective To investigate the clinical significance of mitochondrial DNA (mtDNA) content in peripheral blood mononuclear cells (PBMC) in the diagnosis of primary liver cancer (PLC).Methods The whole blood samples, clinical data and laboratory indexes of 250 PLC patients (test group) and 48 healthy individuals (control group) were collected. The PBMC were isolated by FicollHypaque centrifugation. The gene content of NADH dehydrogenase subunit 1 (ND1) in mitochondria was detected by quantitative real-time polymerase chain reaction, and the β-globin of nuclear genome was amplified simultaneously as the internal reference. The mtDNA content in PBMC were compared between 2 groups, and the diagnostic efficacy of mtDNA, alpha-fetoprotein (AFP) and mtDNA combined with AFP were analyzed by receiver operating characteristic curves.Results The mtDNA content in PBMC of liver cancer patients (90.51) was significantly lower than that of control group (456.67). The area under the curve (AUC) (0.884) and sensitivity (75.20%) of mtDNA were slightly higher than those of AFP (0.866, 71.70%). The AUC of mtDNA combined with AFP in the diagnosis of PLC was 0.957, significantly higher than that of mtDNA and AFP alone. And the sensitivity increased to 84.91% when the 2 markers were combined.Conclusion The content of mtDNA in PBMC can be used as an auxiliary indicator in the diagnosis of liver cancer.

Key words: Primary liver cancer, Peripheral blood mononuclear cell, Mitochondrial DNA

廖新爱, 冯惠娟, 卓传尚, 叶治, 柳丽娟. 原发性肝癌患者外周血单个核细胞线粒体DNA含量检测及其临床意义[J]. 肝脏, 2021, 26(5): 514-517.

LIAO Xin-ai, FENG Hui-juan, ZHUO Chuan-shang, YE Zhi, LIU Li-juan. Detection and clinical significance of mitochondrial DNA content in peripheral blood mononuclear cells of patients with primary liver cancer[J]. Chinese Hepatolgy, 2021, 26(5): 514-517.

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